FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727607
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT19980.27459878753228045No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16690.22938207026595403No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13140.1805919954041124No Hit
GTCTATGGTGGGTCCTTCAGTGCTTACTTCTGGAGCTGGCTCCGCCAGCC13110.18017968491232217No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12100.16629856502205176No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGG10480.14403379846538034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10450.14362148797359015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9950.1367496464437533No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG9940.13661220961315657No Hit
GAGTTACAGTACTCTCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT9900.1360624622907696No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9760.1341383466624153No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9060.12451776852064371No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8660.11902029529677423No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8600.1181956743131938No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAAT8540.11737105332961337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8380.11517206404006558No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8320.11434744305648516No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTCCCACTGAACCT8240.11324794841171128No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG7930.10898740666321242No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7860.10802534884903525No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTC7840.10775047518784178No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7680.105551485898294No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7630.1048643017453103No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7420.10197812830277883No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7400.10170325464158537No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7330.10074119682740819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAC150.00237250168.87247540
ATATCGT554.7293724E-1155.7770337
AATATCG501.2696546E-954.3953836
TCGCGTA408.7833723E-751.2919938
ATCGTAT552.7011993E-949.95716539
GACCGGT200.00860449149.74110410
TCGTATG5100.049.2910940
CGCGTAT352.3330564E-549.06507539
CGTATGC5250.048.73694241
TATCGTA507.4433046E-847.8725238
ATCGCGT507.632116E-847.72035237
ATGCCGT5450.047.4894844
GTCGTAT751.8189894E-1145.7940739
GCCGTCT4550.045.65711246
CCGTCTT4800.044.8870647
CGTCTTC4700.044.5084948
ATCTCGT4600.044.45995337
AGGCAAT6600.043.83296232
AATCTCG4750.043.65944736
CCCACGA7050.042.8238219
Produced by FastQC (version 0.11.5)