FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727607
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51440.706975056589615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16420.2256712758398421No Hit
GTCTATGGTGGGTCCTTCAGTGCTTACTTCTGGAGCTGGCTCCGCCAGCC13570.18650177911977206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11770.16176314961235944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11060.15200513463999107No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10510.14444610895717058No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10410.14307174065120318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10260.14101018819225214No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGG10180.13991069354747826No Hit
GAGTTACAGTACTCTCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT9910.13619989912136635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9900.1360624622907696No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTCCCACTGAACCT9300.1278162524549654No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG9290.12767881562436864No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8830.12135672141691874No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8610.11833311114379053No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAAT8520.1170961796684199No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC8430.11585924819304927No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7990.10981202764679283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7960.10939971715500263No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG7850.10788791201843853No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGAGTGCTGATGGTGAGAGTGAAATCTGTC7790.1070632910348581No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7700.10582635955948747No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7510.10321505977814947No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7320.10060375999681147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC600.060.7256553
TCGCTCT250.00869057949.61470868
CCTAACG851.8189894E-1242.8651662
TACTGAT5350.035.38081770
ATTTTCG552.5235067E-432.75538352
TCTACTG3950.032.44843768
GTATCAA29550.031.9615021
TATCAAC30800.030.7571472
CGTGTCT4150.030.2360464
GTCTACT4050.030.1789567
GACGTGT3900.029.90102462
ACGTGTC3950.029.68475363
AACGCAG31700.029.3594536
CAACGCA31900.029.2792055
AACGCCA1252.0008883E-1029.1463095
TCAACGC32250.028.961454
ATCAACG32450.028.8870053
TCGCTAT705.008022E-528.45184524
TGGATTG9250.028.04257470
GTAGTTT1505.5194134E-528.04257270
Produced by FastQC (version 0.11.5)