FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819355
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30430.37138969067132077No Hit
GAGTTACAGTACCCCGATCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT23670.288885769904376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22250.27155506465451484No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20030.24446058179909808No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18860.23018105705097303No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18800.22944877373055633No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18570.22664168766895915No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17300.2111416907201396No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16030.19564169377132012No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA16010.19539759933118123No Hit
CTGTAACTCTGTTGACAGTAGAAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15960.194787363230834No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAGCTATTTACATTGGTATCAGCAGAAA15340.18722043558652843No Hit
ACAGTACCCCGATCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGA14380.17550390245986172No Hit
GGGTACTGTAACTCTGTTGACAGTAGAAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13980.17062201365708393No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAG13380.16329918045291722No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13290.16220075547229224No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13090.15975981107090334No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13070.15951571663076444No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12560.15329130840722277No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12520.15280311952694497No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12330.15048422234562553No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12200.14889760848472275No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11950.1458464279829866No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11550.14096453918020882No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11360.13864564199888937No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11340.13840154755875048No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11260.13742516979819494No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10960.13376375319611158No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10850.13242123377534767No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10750.13120076157465324No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCCTGGCCGAAGGTG10690.13046847825423658No Hit
GGCCGAAGGTGATCGGGGTACTGTAACTCTGTTGACAGTAGAAAGTTGCA10420.12717320331236154No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10420.12717320331236154No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10270.1253424950113199No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCCTGGCCGAAGGTGATCGGGGTA10220.12473225891097267No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATG10030.12241336172965321No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTTCTACTGTCAACAGAGTTACAGTACC9970.12168107840923653No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9960.12155903118916708No Hit
CCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA9700.11838580346736152No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8810.10752360088118094No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8770.10703541200090315No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8660.10569289258013925No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8560.1044724203794448No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA8520.10398423149916704No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA8520.10398423149916704No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATGTAAAT8480.10349604261888924No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8440.10300785373861146No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT8420.10276375929847259No Hit
GAGTATTAGCAGCTATTTACATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATC8280.10105509821750035No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8280.10105509821750035No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8220.10032281489708368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA200.007884270550.8540428
GTGGTAT2550.037.1537061
GTATCAA22500.036.5433241
ACGGGTT400.002862082633.81987816
ATCAACG25550.031.6203693
TCAACGC25450.031.6137184
CAACGCA25850.031.1245335
TATCAAC26200.030.9743672
AACGCAG26050.030.8855726
TTGCCGA703.130096E-530.48135656
CCGTTAA450.005094620430.06211716
GTCGGGA902.3779467E-730.0566082
AATGGTA5500.029.61499870
GACAGGT1152.0954758E-929.4229621
GGCGCGT704.2203763E-529.17554732
CGCAGAG27800.028.9413388
ACGCAGA27900.028.8376057
TAGTTCG500.006597033728.51334857
AGAGTAC28250.028.2358211
CCGCATT606.077456E-428.19356221
Produced by FastQC (version 0.11.5)