FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726561
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80540.46647642336413253No Hit
GATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA75260.43589540132089166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC64100.37125824109313255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54130.31351339454557353No Hit
CCATACTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG52170.3021613484840675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47960.27777761689277125No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47560.27546087279858633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45250.26208167565466844No Hit
GCATATATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT45170.26161832683583147No Hit
GAGTTACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGAACTGTGGCTGCACCAT43670.252930536482638No Hit
GTCTCCATACTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCAC43420.25148257142377245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42760.24765994366836735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39430.22837304908427794No Hit
GTTACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAA37360.216383898396871No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG37240.2156888751686155No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37050.2145884217238777No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36220.209781177728444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36090.2090282358978339No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCACCTATTTAAATTGGTATCAGCAGAAA35950.20821737546486918No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35740.20700108481542212No Hit
GTCCTGATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT35570.20601646857539352No Hit
TATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT34790.20149881759173294No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC33810.19582279456097992No Hit
ACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGA33710.19524360853743367No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33650.19489609692330592No Hit
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA33210.19234767841970252No Hit
GTATATTACTGTGCGAGAGAATTCGATATACAGAAATACAGTAGTGCCAA33210.19234767841970252No Hit
GTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCCCCTGGCCGAACGTC32900.1905522017467092No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31730.18377572527121833No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30710.17786802783104683No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29620.1715549001743929No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT29360.17004901651317272No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29200.16912231887549875No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC29180.16900648167078952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28950.16767435381663318No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28800.16680557478131383No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGAA28700.1662263887577676No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTG28640.16587887714363986No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCC27810.16107163314820616No Hit
GGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT27280.15800194722341115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27120.1570752495857372No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26960.15614855194806324No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACC26660.15441099387742455No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26000.15058836612201945No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA25790.14937207547257236No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25100.14537569191010338No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC25000.14479650588655715No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA24790.14358021523711006No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24090.13952591307228646No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24020.1391204828558041No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23630.13686165736397382No Hit
CCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC23300.13495034348627127No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23230.1345449132697889No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22450.13002726228612832No Hit
TCCATGGGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA22160.12834762281784426No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21920.1269575763613333No Hit
CCCCTAAGGTCCTGATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA21570.12493042527892151No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATACTCC21540.12475666947185764No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21220.12290327419650972No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA21090.12215033236589962No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20790.12041277429526093No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20160.11676390234691969No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19970.11566344890218186No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19680.11398380943389778No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG19230.11137747232793976No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCATGGACGTT19180.11108787931616664No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC19010.11010326307613807No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18750.10859737941491786No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18710.10836570500549939No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18650.10801819339137163No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18620.10784443758430776No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18370.1063964725254422No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18270.10581728650189597No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18170.10523810047834974No Hit
CTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAA18160.10518018187599511No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18050.10454307725009425No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18030.10442724004538502No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17930.10384805402183879No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17640.10216841455355473No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17590.1018788215417816No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17580.101820902939427No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATATATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG17530.10153130992765387No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT17440.10101004250646227No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17430.10095212390410764No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCGCCGGGAAGGGA17380.10066253089233454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT801.0119347E-650.2517770
ATGCCGT758.3491614E-1041.58334744
TCGTATG602.9261173E-740.22463640
CGTATGC801.6316335E-938.83580441
GTATCAA37450.036.8876531
CCCACGA1750.035.0771419
TGTTAGA19750.034.264570
TATCAAC40750.033.6424942
ACGAGAC1850.033.18684822
GTACGTA801.8816634E-532.84025269
ATCAACG42150.032.605983
TCTACTG12500.032.47120768
CTAGAGA3700.032.259384
TCAACGC42600.032.181494
CAACGCA43450.031.6304265
AACGCAG43750.031.335586
AATGGTA9200.031.3163270
GTGCTAG3500.031.1860071
GTCTACT12250.031.17633267
CGTGTCT12100.031.1638864
Produced by FastQC (version 0.11.5)