FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726561
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81050.4694302720842183No Hit
GATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA70140.4062410769153247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54700.31681475487978705No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53050.30725818549127426No Hit
CCATACTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG49360.2858862212224184No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48670.28188983765994946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47070.2726228612832098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45520.2636454779182432No Hit
GAGTTACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGAACTGTGGCTGCACCAT44480.25762194327336246No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43470.25177216443554556No Hit
GTCTCCATACTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCAC42780.2477757808730766No Hit
GCATATATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT41810.24215767644467817No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCACCTATTTAAATTGGTATCAGCAGAAA38810.2247820957382913No Hit
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA37250.21574679377097017No Hit
GTCCTGATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT36690.21250335203911128No Hit
TATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT35030.20288886404824388No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34770.20138298038702368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34410.19929791070225725No Hit
GTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCCCCTGGCCGAACGTC34250.1983712130645833No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG34220.19819745725751944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33980.19680741080100847No Hit
GTTACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAA33540.19425899229740506No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC32540.18846713206194277No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32390.18759835302662345No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31410.1819223299958704No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31180.18059020214171406No Hit
GTATATTACTGTGCGAGAGAATTCGATATACAGAAATACAGTAGTGCCAA31120.18024269052758635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30950.17925807428755774No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30880.17885264407107537No Hit
ACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGA30720.17792594643340143No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT30430.17624630696511737No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30410.17613046976040814No Hit
GGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT29280.16958566769433575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29170.1689485630684349No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27980.16205624938823476No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27920.16170873777410702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27660.16020285411288684No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTG27540.15950783088463136No Hit
TTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC27250.1578281914163473No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA26940.15603271474335398No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACC26870.1556272845268716No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26710.15470058688919766No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26480.15336845903504134No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGAA26360.15267343580678586No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGTTTCCACCTTGGTCC25950.1502987731102463No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC25280.14641822675248659No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA23720.13738292478516542No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23260.13471866907685276No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23190.13431323886037042No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23050.1335023784274057No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23010.1332707040179872No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA22930.1328073551991502No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22760.1318227389591216No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22590.13083812271909304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22430.12991142508141906No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21400.1239458090388929No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATACTCC20750.12018109988584244No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20710.11994942547642394No Hit
TCCATGGGGTATTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA20610.11937023945287771No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20600.1193123208505231No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT20440.11838562321284912No Hit
CCCCTAAGGTCCTGATATATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA20360.11792227439401215No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20350.11786435579165752No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20340.11780643718930289No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20110.11647430933514656No Hit
CCCCATGGACGTTCGGCCAGGGGACCAAGGTGGAAACCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC20040.1160688791186642No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAATACCCCATGGACGTT19340.11201457695384062No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18290.10593312370660521No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18040.10448515864773965No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18010.10431140284067578No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC17940.10390597262419342No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17700.10251592616768246No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17510.10141547272294463No Hit
CTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAA17420.10089420530175301No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17270.10002542626643368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT851.1950306E-649.05379570
AATCGAG700.001301277739.71021770
GTCTGGG14800.037.56371770
GTATCAA36450.037.0314331
ATGGCAA5500.036.641770
GAGTTTA4600.036.308741
AATGGTA9000.035.51858570
TATCAAC39600.033.63882
ATCAACG40600.032.9752243
CAACGCA41350.032.2928665
AACGCAG41500.032.258236
TCAACGC41350.032.2095534
CGTGTAT450.003805735831.91062764
AAAACCG2850.031.69850770
GACAGGT1550.030.7871321
GTGGTAT4300.030.1222921
AGAGTAC44500.030.00696811
ACGCAGA44800.029.8820657
TATATAC803.0491647E-629.8077553
CGCAGAG44800.029.8060288
Produced by FastQC (version 0.11.5)