FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880741
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22250.2526281846763123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21720.24661052454694402No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18150.20607647424157613No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17550.19926402881210253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17180.19506302079726048No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17090.19404115398283944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15470.17564755132326076No Hit
GTAATACACAGCCGTGTCCTCGGGAGTAACAGAGTTCAGTTGCAGGGAGA14500.16463409787894512No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13850.15725394866368206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13410.15225815534873477No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA12640.14351551704757698No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12050.13681661237526127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11810.13409163420347184No Hit
GTTTATTACTGTCAACACCATGGTGGCTCAGTATCCACTTTCGGCCCTGGGACCAAAGTGGACATCAAACGAA11790.13386455268915606No Hit
GAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTTTA11530.13091249300305083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10930.12410004757357725No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10520.11944487653010363No Hit
CATCTATGGTGCATCCAGAAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGGAGTGGGTCTGGGACAGAGTTCA10520.11944487653010363No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10510.11933133577294573No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10400.11808238744420892No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10390.11796884668705103No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10150.11524386851526158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10130.11501678700094581No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10010.11365429791505108No Hit
GTCCCAGACCCACTCCCACTGAACCTGTCTGGGATGCCAGGGGCCCTTCT9830.111610564286209No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9820.11149702352905111No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9470.10752309702852483No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9450.10729601551420906No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9000.10218668144210385No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACTTTGGTCCCAGGGCCGAAAGTG8890.10093773311336705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)