FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880741
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33740.3830865146507316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22130.2512656955904176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19110.21697638692873386No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18060.2050546074271551No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17590.19971819184073414No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15880.1803027223667344No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14660.1664507499934714No Hit
GTAATACACAGCCGTGTCCTCGGGAGTAACAGAGTTCAGTTGCAGGGAGA13560.15396126670610316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12940.1469217397623138No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12720.14442384310484013No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA12450.141358242661577No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12200.13851972373262966No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12050.13681661237526127No Hit
GTTTATTACTGTCAACACCATGGTGGCTCAGTATCCACTTTCGGCCCTGGGACCAAAGTGGACATCAAACGAA12010.1363624493466297No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11870.1347728787464192No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11450.1300041669457877No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT11260.12784689255978773No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11010.12500837363084039No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10510.11933133577294573No Hit
GAGTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTCTGCAGTTTA10410.1181959282013668No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10300.11694697987262997No Hit
CATCTATGGTGCATCCAGAAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGGAGTGGGTCTGGGACAGAGTTCA10290.11683343911547209No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10010.11365429791505108No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9980.11331367564357739No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9830.111610564286209No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9260.10513874112820909No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9170.10411687431378805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9170.10411687431378805No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9100.1033220890136828No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9070.10298146674220911No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8900.10105127387052494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT150.002510312567.90008529
CGCCTAT252.6891538E-454.6822136
CCTAACG652.6375346E-1046.7648052
CTAACGC652.6375346E-1046.7648053
TCTATCG306.780771E-445.3340231
GGCGTTG5200.044.51357770
GGTATCA12950.041.7529221
GTATCAA32600.038.562221
CGCATAA1106.5964286E-638.259670
CGGCGTT4750.033.9891869
TATCAAC38150.032.490882
TGGTATC4500.032.2734872
GTGCTAG951.0755684E-832.0151521
CAACGCA38800.031.9429455
AACGCAG39400.031.6279266
CGCAGAG40600.030.5267568
ACGCAGA41300.030.0093527
AGAGTAC42150.029.484311
GCAGAGT42200.029.369349
ATCAACG43550.028.3846073
Produced by FastQC (version 0.11.5)