FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662712
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20820.3141636185854489No Hit
GAGTTACACTCCCCCTTGGACGTTCGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT18390.2774961069061674No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16490.24882603604582382No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC14690.2216649162833931No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14270.21532732167215923No Hit
GATCTATGGTGTATTCAATTTGCAAAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA12820.19344753075242338No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12780.19284395031325827No Hit
ACATAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGTATTCAATTTGCAAAGTGGGGTCCCATTAAGGTT12590.1899769432272239No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12200.18409203394536391No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12050.18182860729849468No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAACATAAA12030.1815268170789121No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTCCC11490.17337848115018287No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11390.17186953005227007No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTATGTTGATACCAATT11320.1708132642837311No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10840.16357029901374956No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10610.16009971148855007No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9770.14742452226608238No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9710.1465191516073347No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG9680.14606646627796088No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9500.1433503543017178No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9460.14274677386255266No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9120.13761634012964907No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9080.13701275969048393No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8890.1341457526044496No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8810.13293859172611935No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8800.13278769661632805No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTAATGGGACCCCA8490.1281099482127983No Hit
GGTTTATGTTGATACCAATTTAAATAGCTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT8450.1275063677736332No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC8290.12509204601697269No Hit
GTCCTGATCTATGGTGTATTCAATTTGCAAAGTGGGGTCCCATTAAGGTT8130.12267772426031218No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8010.1208669829428168No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7880.11890534651553013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7800.11769818563719986No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCGAACGTC7800.11769818563719986No Hit
GTCCAAGGGGGAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTC7520.11347312256304398No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7510.11332222745325271No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7400.11166238124554859No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7000.10562657685389731No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTATGTTGATACCAATTTAAAT6920.10441941597556707No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG6840.1032122550972368No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT6840.1032122550972368No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCCTGGACGTGTCTACTGA6830.10306135998744553No Hit
CCTTAGGGGCTTTCCCTGGTTTATGTTGATACCAATTTAAATAGCTGCTA6800.10260867465807168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6680.10079793334057631No Hit
ACTTACTACTGTCAACAGAGTTACACTCCCCCTTGGACGTTCGGCCAGGGGACCAAGGTGGACATCAAACGAA6660.10049614312099373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG700.052.993762
TACGGAT200.00806030450.5696877
CTAACGC701.0913936E-1148.1761443
CGTGAAA451.4333782E-647.7733257
GTCGTAT605.7680154E-945.8503339
ACGAGAC1750.044.32205622
AATGTCG651.232911E-842.0740436
CCCACGA1850.041.91993719
CTTATAC1850.040.1033821
TACGTAT700.001387398139.18868670
ATCTCGT702.331035E-839.14362737
ATGTCGT702.331035E-839.14362737
CGAGCCC2000.038.77009215
ATCTCCG2000.038.76716610
CGAGACA2100.036.93504323
GCCCACG2150.036.065218
TCGTATG1151.8189894E-1235.9603640
ATGCCGT904.798494E-934.77313244
TTCGATC807.2921466E-834.3877539
CACGAGA2300.033.723321
Produced by FastQC (version 0.11.5)