FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662712
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20730.31280556259732734No Hit
GAGTTACACTCCCCCTTGGACGTTCGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT17940.27070582696555967No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15820.23871606368980797No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC14420.21759074831902847No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14100.21276210480570745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13620.20551913953572593No Hit
GATCTATGGTGTATTCAATTTGCAAAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA12950.19540916717971005No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12110.18273397795724236No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12080.18228129262786852No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTCCC11950.18031965620058185No Hit
ACATAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATGGTGTATTCAATTTGCAAAGTGGGGTCCCATTAAGGTT11780.17775443933413004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11440.17262400560122648No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11400.17202042516206137No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAACATAAA11150.16824804741727928No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10920.16477745989207981No Hit
CCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTATGTTGATACCAATT10770.1625140332452106No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG9820.1481789978150388No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9530.14380303963109164No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9450.1425958787527614No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9350.14108692765484857No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9180.13852171078839676No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9040.13640917925131885No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8880.1339948574946583No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTAATGGGACCCCA8660.13067516507925012No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8620.13007158464008498No Hit
GGTTTATGTTGATACCAATTTAAATAGCTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT8550.12901531887154602No Hit
GTCCTGATCTATGGTGTATTCAATTTGCAAAGTGGGGTCCCATTAAGGTT8540.12886442376175472No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8500.1282608433225896No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8190.12358309491905986No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8180.12343219980926858No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCCTGGACGTGTCTACTGA7870.11875445140573884No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCGAACGTC7770.11724550030782603No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7750.11694371008824345No Hit
GTCCAAGGGGGAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTC7620.11498207366095681No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7500.11317133234346141No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC7390.11151148613575732No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7340.1107570105868009No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7320.11045522036721835No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7000.10562657685389731No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6960.10502299641473219No Hit
CCTTAGGGGCTTTCCCTGGTTTATGTTGATACCAATTTAAATAGCTGCTA6860.10351404531681936No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG6770.10215598932869782No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6770.10215598932869782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGA150.002582412467.4150161
TACGTAT750.001751221737.37423370
TAACCGT501.5955242E-435.41360553
AATGGTA4300.034.223570
TAACGGC601.42636945E-534.19291336
TTAACGG601.4429121E-534.1353935
AAGCGAG400.002916301633.6896977
GGTCGTA450.004366932431.0234447
GTGGTAT3400.029.7419191
ATGCACG500.00680615928.33088353
ATCAACG22650.028.2542763
AACGCAG23050.028.2087276
TCAACGC22700.028.1962974
TATTAGA1203.4542609E-928.0747492
CGCTTCT1700.027.76331717
CAACGCA23150.027.6523785
AACGGCC756.478166E-527.39630137
ACGGGCT500.00865444326.97007816
CGCAGAG24200.026.8682338
ACGCAGA24400.026.7860727
Produced by FastQC (version 0.11.5)