FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452289
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58480.4026746742556061No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50500.3477269331379636No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAATTGTTGCTGCCTGCATATGAGCTG49950.3439398081235897No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45400.31260995573195144No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAAT43060.29649746021625173No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG39290.27053843966318No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC39030.26874816238365784No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT38040.26193133735778484No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG35050.2413431486432797No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34930.24051686682196172No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33040.22750292813620432No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT33000.22722750086243163No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31110.2142135621766742No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30370.2091181576118803No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29670.204298180320859No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29330.20195704849379154No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28960.19940934621139458No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28200.19417622800971432No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGGACCCAGGCAAAGC27920.1922482370933058No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT27690.19066453026911312No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27330.1881856848051593No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26670.18364113478791066No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGGA26540.18274599614814957No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG26540.18274599614814957No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26480.18233285523749063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26030.17923429840754837No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25860.17806373249401464No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC25670.17675545294359454No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24640.16966320064394896No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23420.16126266879388332No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23370.1609183847016675No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22880.1575444005979526No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22790.15692468923196418No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22780.156855832413521No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGGACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG22730.1565115483213052No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22580.15547869604465778No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTGGTGTTCGGCGGAGGGACCAAGCTGAC21070.14508131645974046No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20780.14308446872488878No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19810.13640535733590214No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTCCTGTTGGTACCAGGAGA19730.13585450278835687No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC19470.13406422550883468No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAATTGTTGCTGC18760.12917539139937023No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA18720.1288999641255976No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17910.12332256183170154No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG17870.1230471345579289No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT17660.12160114137062253No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17140.11802058681157815No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16790.1156105981660675No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16720.11512860043696538No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16640.11457774588942007No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16620.11444003225253377No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT16580.11416460497876112No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16380.11278746860989788No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAATTGTTG16270.11203004360702312No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16130.11106604814881886No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC15990.11010205269061461No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15980.11003319587217145No Hit
CAACAGGACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG15930.10968891177995563No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15880.10934462768773982No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG15880.10934462768773982No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15740.10838063222953559No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15690.10803634813731978No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG15430.1062460708577976No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT15430.1062460708577976No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15390.10597064358402494No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15380.10590178676558179No Hit
GTACCAACAGGACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA15150.10431807994138907No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG15110.10404265266761643No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC15020.10342294130162798No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15010.10335408448318482No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC14680.1010818094745605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTT200.006288863753.8483959
CGTATGC3700.048.46752541
TCGTATG3700.048.38939740
ACGAGAC5150.045.99664722
GGTATCA14800.045.2729531
ATGCCGT3400.043.8770844
CGAGACA5400.043.8671723
GGCAATC4000.043.42466433
GTATGCC4150.043.30624442
AGGCAAT5050.043.1184932
CCTAACG551.7118509E-743.0666162
CCCACGA5350.043.00319719
TCTCCGA5450.042.20978511
CACGAGA5500.041.83758521
ATCTCCG5600.041.68470810
ATCTCGT3650.041.2901837
GCCGTCT3550.040.23191546
AATCTCG4000.040.19295536
CTAACGC603.3890865E-739.4777343
TCCGAGC5850.039.32364713
Produced by FastQC (version 0.11.5)