FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201882
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC82080.6829289397794459No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC53870.4482137181520316No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51140.42549934186550764No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44920.37374717318339074No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44770.372499130530285No Hit
CCCCTGTGCTGGTCATCTCTGAGGACAGCAAACGACCCTCCGGGATCCCT38380.31933251350798164No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTATCA36960.30751770972524756No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35630.2964517315343769No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG35630.2964517315343769No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35450.29495408035065007No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34890.29029472111238874No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT32020.26641550501629946No Hit
CTACTACTGTTACTCAACAGACAGTAGTGATAATCATGGGGTGTTCGGCG31580.2627545799005227No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC31280.2602584945943113No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31230.25984248037660934No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG30920.25726319222685756No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30510.2538518756417019No Hit
GTGCTGGTCATCTCTGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG29620.24644682256660805No Hit
TCTCTGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC29230.24320191166853317No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28610.23804333536902958No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28330.2357136557498989No Hit
CCCCATGATTATCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA28030.2332175704436875No Hit
ATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27640.22997265954561263No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26820.22315002637530143No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26520.22065394106908998No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26330.21907308704182277No Hit
GACTACTACTGTTACTCAACAGACAGTAGTGATAATCATGGGGTGTTCGG25630.21324888799399608No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25330.21075280268778468No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA24510.20393016951747345No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24120.20068525861939857No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23860.19852198468734866No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23640.19669152212946028No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23250.19344661123138546No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23170.19278098848306238No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCACGGT22860.1902017003333106No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22620.18820483208834146No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22500.1872063979658569No Hit
GGCCAGGCCCCTGTGCTGGTCATCTCTGAGGACAGCAAACGACCCTCCGG21950.18263024157113594No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20560.171065046319023No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCACGGTGG20180.16790333826448853No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT20050.16682170129846358No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTATCAC19280.16041508234585425No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG18650.15517330320281025No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18530.15417486908032568No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18320.15242760936597768No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17500.14560497619566648No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17260.1436081079506973No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17150.14269287667175312No Hit
CCTCAGAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCAT17100.14227686245405122No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC16970.14119522548802627No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC16700.138948748712436No Hit
GCTCAGGGACAATGGCCACCGTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA16490.13720148899808798No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16420.13661906909330535No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16370.13620305487560344No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15670.13037885582777678No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15640.13012924729715564No Hit
GGTCATCTCTGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC15580.12963003023591335No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15510.12904761033113069No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC15260.12696753924262116No Hit
TATCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCACGGTGG15000.12480426531057125No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14870.1237226283445463No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGTAGTGATAATCATGGGGTGTTCGGCGGAGGGACCAAG14720.1224745856914406No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14380.11964568901106765No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA14340.11931287763690612No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14320.11914647194982536No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14140.1176488207660985No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13700.11398789565032176No Hit
GGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCT13680.11382148996324098No Hit
CTGATAGTCACGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG13630.11340547574553908No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13500.11232383877951412No Hit
TCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCTG13450.11190782456181224No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13260.11032697053454499No Hit
GTAGTGATAATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA13240.11016056484746423No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13190.10974455062976232No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13190.10974455062976232No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG13180.10966134778622194No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTAT13110.10907892788143927No Hit
GCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG13010.10824689944603547No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACT12910.10741487101063167No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG12850.10691565394938937No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12830.10674924826230862No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC12760.10616682835752594No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12360.10283871461591071No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12340.10267230892882995No Hit
AGTCAGGCCAGGCCCCTGTGCTGGTCATCTCTGAGGACAGCAAACGACCC12230.10175707764988576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT354.280082E-968.7543837
AATCGCG501.1586963E-954.96895236
TCGAATC200.007135207852.1559347
CGCGTAT408.364441E-751.6610439
CGCTCAT2250.050.12578628
CGAGACG2200.048.10793323
ACGCTAG306.5235427E-445.6926433
AGACGCT2350.045.05408525
ACGAGAC2350.045.0315922
CACGAGA2200.044.99670421
TATACAC2750.044.686383
CGAGCCC2300.044.5152115
GAGACGC2400.044.10260824
CCCACGA2350.043.57714519
GGTATCA10550.043.360461
CGTATGC803.45608E-1143.1232941
ATCTCCG2650.042.49765810
GACGCTC2500.042.3543726
TCTCCGA2700.040.4467111
TCGCGTA602.9824696E-740.12521738
Produced by FastQC (version 0.11.5)