FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201882
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC87750.7301049520668419No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49980.41584781201482346No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC49640.4130189153344505No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47200.3927174215105976No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41790.34770468315525155No Hit
CCCCTGTGCTGGTCATCTCTGAGGACAGCAAACGACCCTCCGGGATCCCT35590.2961189201602154No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34710.2887970699286619No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG33870.2818080310712699No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTATCA33630.27981116282630075No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33340.27739828036362973No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32730.2723229069076665No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32580.2710748642545608No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC31130.25901045194120553No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30980.2577624092880998No Hit
GTGCTGGTCATCTCTGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG30700.2554327296689692No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30130.2506901675871675No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT29690.24702924247139071No Hit
CTACTACTGTTACTCAACAGACAGTAGTGATAATCATGGGGTGTTCGGCG29270.24353472304269472No Hit
TCTCTGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC29120.242286680389589No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29030.24153785479772558No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG28920.24062262351878136No Hit
CCCCATGATTATCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA28450.23671208987238349No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27980.23280155622598558No Hit
ATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC26980.2244812718719475No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26760.22265080931405912No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25130.20908874581697703No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25070.20858952875573478No Hit
GACTACTACTGTTACTCAACAGACAGTAGTGATAATCATGGGGTGTTCGG23800.19802276762610638No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23210.19311379985722393No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23010.1914497429864163No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22700.1888704548366645No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21790.18129899607448985No Hit
GGCCAGGCCCCTGTGCTGGTCATCTCTGAGGACAGCAAACGACCCTCCGG21720.18071657616970718No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA21620.17988454773430337No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21240.1767228396797689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20750.17264590034629024No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCACGGTGG20440.17006661219653843No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCACGGT20400.1697338008223769No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20360.16940098944821538No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19320.16074789372001577No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG18870.15700376576069866No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC18670.15533970888989101No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT18510.15400846339324492No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18270.15201159514827578No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17990.14968191552914512No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17570.14618739610044912No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTATCAC17320.1441073250119396No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17220.1432752965765358No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC17080.14211045676697048No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16590.13803351743349182No Hit
GGTCATCTCTGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC16520.13745109752870913No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16270.1353710264401996No Hit
GCTCAGGGACAATGGCCACCGTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAA16020.13329095535169008No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15930.13254212975982668No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15860.131959709855044No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15670.13037885582777678No Hit
GGATGAAGCTGACTACTACTGTTACTCAACAGACAGTAGTGATAATCATGGGGTGTTCGGCGGAGGGACCAAG15600.1297964359229941No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15590.12971323307945373No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA15390.12804917620864611No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC15380.12796597336510573No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15320.12746675630386342No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15310.12738355346032307No Hit
CCTCAGAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCAT14910.12405543971870782No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG14830.12338981697038477No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14700.12230818000435982No Hit
GTAGTGATAATCATGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA14290.1188968634192042No Hit
TATCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCACGGTGG14200.11814803782734078No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATGATTAT14110.11739921223547736No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14030.11673358948715432No Hit
GGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCT13780.1146535183986448No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13630.11340547574553908No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13510.11240704162305451No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13470.11207423024889299No Hit
TCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCCTCCACCTG13460.11199102740535259No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13300.11065978190870651No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12990.1080804937589547No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACT12890.1072484653235509No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12860.10699885679292975No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC12760.10616682835752594No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12690.10558440845274328No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC12680.1055012056092029No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12620.10500198854796063No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12600.10483558286087985No Hit
CTGATAGTCACGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG12530.10425316295609718No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12500.10400355442547606No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12290.10225629471112806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG150.001466823977.7498168
CGTAATC200.002667006666.87118569
GTAACGT200.007716955651.1322068
GGTATCA13600.044.624641
GTATCAA35800.038.1904641
TATCAAC38100.035.2527162
GTGGTAT3200.034.095231
ATCAACG39050.034.0416563
TCAACGC39500.033.653844
AATGGTA6550.033.59907570
AACGCAG40050.033.53356
CAACGCA39800.033.485815
ATCGTTG958.898496E-932.6458740
TTAATCG959.1749826E-932.54319837
GTGCTAG2150.031.7164941
GAATACG2050.031.5938829
CGCAGAG42550.031.4844578
ACGCAGA43000.031.3135197
AGAGTAC43700.030.88865311
AATACGT2100.030.84164610
Produced by FastQC (version 0.11.5)