FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309795
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTGGCTTGGTACCAGCAGAG73290.5595532125256242No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47460.3623467794578541No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT46760.35700243167823975No Hit
ATATTACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT41740.3186758233158624No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAGAGACAGCCAGGGAGTCTGGAGA39050.29813825827705864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36880.2815707801602541No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33370.2547726934367592No Hit
GTAATACATAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA33090.25263495432491345No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32520.2482831282757989No Hit
GCTTAGGAGGCTGTCCCGGTCTCTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT30950.23629651968437806No Hit
ACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA27210.20774243297615275No Hit
CTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27180.20751338949988357No Hit
GTTTATTACTGTCAGCAATATTACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26480.20216904172026923No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26380.20140556346603858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25770.19674834611523176No Hit
TTACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT24680.18842643314411797No Hit
GTACCAGCAGAGACCGGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA24130.18422730274584956No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23140.17666886802896636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22010.1680415637561603No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21840.16674365072396827No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21840.16674365072396827No Hit
ATGTATTACTGTGCGAGAGATGGGAGGGGTAGTACCAGTGCGAACAACTGGTTCGACCCCTGGGGCCAGGGAACC21030.1605594768647002No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20800.15880347687996976No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20780.15865078122912366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20580.1571238247206624No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTA20520.156665737768124No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20280.15483338995797052No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20040.15300104214781704No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19720.15055791173427904No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19720.15055791173427904No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19540.1491836508766639No Hit
GTCTCCAGACTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA19410.14819112914616409No Hit
GACCGGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA19330.1475803465427796No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18900.14429739004958791No Hit
GTACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18710.14284678136654974No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18400.1404799987784348No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18110.138265911841166No Hit
GAGTACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18080.13803686836489681No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17590.13429582491916672No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17400.13284521623612855No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA17150.130936520600552No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTACT16540.12627930324974518No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT16500.12597391194805294No Hit
CTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA16500.12597391194805294No Hit
CTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAC15870.12116399894640001No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCCCTGTATCTGCAAATGAA15740.12017147721590019No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15710.11994243373963101No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15440.11788104245320832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15340.1171175641989777No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.11475078161086276No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14870.11352921640409377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14620.11162052076851721No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14030.10711599906855654No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13790.10528365125840303No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13740.10490191213128773No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13650.10421478170248015No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCCGGTCTCTGCTGGTACCAAGCCA13570.10360399909909565No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13290.10146625998724991No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13260.10123721651098072No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13170.10055008608217317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)