FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309795
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTGGCTTGGTACCAGCAGAG60020.45823964818922047No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49540.3782271271458511No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT39780.30371164953294216No Hit
ATATTACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT39710.3031772147549807No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAGAGACAGCCAGGGAGTCTGGAGA34490.2633236498841422No Hit
CTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34330.26210208467737317No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31660.24171721528941553No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30430.23232643276237885No Hit
GTAATACATAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA29620.22614225890311082No Hit
GCTTAGGAGGCTGTCCCGGTCTCTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT29190.2228593024099191No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28630.21858382418622763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28600.21835478070995842No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28300.21606434594726656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27300.2084295634049603No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26690.2037723460541535No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25290.1930836504949248No Hit
GTTTATTACTGTCAGCAATATTACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24640.18812104184242573No Hit
ACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA22780.17392034631373612No Hit
GTACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22530.17201165067815957No Hit
TTACAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT22500.17178260720189037No Hit
GAGTACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT21830.1666673028985452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21570.16468225943754558No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20720.15819269427658525No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20700.15803999862573914No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTA20100.1534591291003554No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19810.1512450421630866No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19570.14941269435293308No Hit
ATGTATTACTGTGCGAGAGATGGGAGGGGTAGTACCAGTGCGAACAACTGGTTCGACCCCTGGGGCCAGGGAACC19250.1469695639393951No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18950.14467912917670323No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18720.1429231291919728No Hit
GTACCAGCAGAGACCGGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA18660.1424650422394344No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18580.14185425963604992No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18390.14040365095301174No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18300.13971652052420416No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18010.13750243358693537No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17930.13689165098355086No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17520.1337613901412053No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17060.13024939017174442No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17060.13024939017174442No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17060.13024939017174442No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTACT17010.12986765104462913No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16360.12490504239213007No Hit
GACCGGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA15910.12146939024809227No Hit
CTGCTGGTACCAAGCCAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAC15870.12116399894640001No Hit
GTCTCCAGACTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA15840.12093495547013083No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT15550.11872086853286201No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA15160.11574330334136258No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14790.11291843380070926No Hit
CTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA14090.1075740860210949No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14080.10749773819567184No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14070.10742139037024877No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14010.1069633034177104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13910.10619982516347978No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13870.10589443386178753No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13850.10574173821094142No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCCCTGTATCTGCAAATGAA13800.1053599990838261No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13740.10490191213128773No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13410.10238243389232667No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCCGGTCTCTGCTGGTA13220.10093182520928846No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13180.10062643390759622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)