FastQCFastQC Report
Wed 8 May 2024
EGAF00004692947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662743
Total Bases48.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14820.22361609251248224No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14730.22225810004783153No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11390.1718614908041277No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11220.16929639392645413No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11180.16869284171994273No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10650.16069577498366636No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10610.16009222277715496No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG10480.15813067810599282No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10320.15571646927994712No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10310.15556558122831926No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10170.1534531485055293No Hit
GTCTATTACTGCATGCAAGTTACACAATTTCCTCGGACTTTTGGCCAGGG9770.14741762644041506No Hit
CTATTACTGCATGCAAGTTACACAATTTCCTCGGACTTTTGGCCAGGGGA9710.14651229813064792No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9580.14455075345948581No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT9450.14258920878832368No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9260.1397223358073944No Hit
CTCCTGTACAGCTTCTGGATTCAACTTTGGTGATTATACTATGAGCTGGT8050.12146488156042387No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7960.12010688909577316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC7960.12010688909577316No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7550.11392047897903108No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7500.11316603872089182No Hit
GTAATAGACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGA7420.11195893430786896No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7310.11029916573996254No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC7110.10728140470740544No Hit
CAGTAATAGACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGT6950.10486719588135975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6820.10290565121019762No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6630.10003877822926836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)