FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686003
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69460.41198028710506446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60920.36132794544256447No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51490.3053968468620756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48700.28884883360231267No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42400.2514823520480094No Hit
CATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATCGGAACATACTACTGTCAACACTATGATAGCCTC40260.23878961069464288No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38840.23036732437605392No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG38800.23013007687412182No Hit
GTTCAGTGGAGGTGGATCTGGGACACATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATCGGAACATA38100.22597824559031035No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37820.22431751307678574No Hit
CTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT37570.22283471618971024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37410.22188572618198188No Hit
ATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA36810.2183270136530006No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG35190.20871848982475122No Hit
GAGTAAAATGTGTCCCAGATCCACCTCCACTGAACCTCGCTGGGACCCCT34740.20604945542801525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34630.205397024797702No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31720.18813726903214287No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31280.18552754651088996No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31070.18428199712574653No Hit
GTCCAGGGGAGGCTATCATAGTGTTGACAGTAGTATGTTCCGATATCTTC31010.1839261258728484No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30800.18268057648770494No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC30680.1819688339819087No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30020.1780542502000293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29800.17674938893940284No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGCACATTAGCAA29760.17651214143747074No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29230.17336861203687065No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28980.17188581514979512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28220.1673781126130855No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28220.1673781126130855No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGC28060.16642912260535717No Hit
CCACTGAACCTCGCTGGGACCCCTGTTTCCAAATTGGCAGCATCGTAGAT27950.16577669197504394No Hit
ATACTACTGTCAACACTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGA27450.16281109820089287No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC24410.14478028805405446No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24030.14252643678569968No Hit
GTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATGTGTCCCAGATCCACCTCCA23950.1420519417818355No Hit
CATTAGCAACTATGTAAATTGGTATCAGCAGATGTCAGGGAAAGCCCCCA23940.14199262990635247No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT23820.14128088740055625No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT23630.14015396176637882No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGCACATTAGCAA23310.13825598175092216No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23090.1369511204902957No Hit
GGCTATCATAGTGTTGACAGTAGTATGTTCCGATATCTTCAGGCTGCAGG23040.13665456111288057No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22550.13374827921421253No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22180.13155373982134078No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22090.13101993294199357No Hit
ACATACTACTGTCAACACTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21630.1282915866697746No Hit
CTATCATAGTGTTGACAGTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATGTGTC21500.12752053228849533No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21210.12580048789948772No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21160.12550392852207262No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21040.12479218601627637No Hit
CAGTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATGTGTCCCAGATCCACCTCCA20650.12247902287243854No Hit
ATCATAGTGTTGACAGTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGC20100.11921686972087239No Hit
CACTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20070.11903893409442332No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20010.11868306284152519No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT18750.11120976653066453No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18720.11103183090421548No Hit
CTCCTGATCTACGATGCTGCCAATTTGGAAACAGGGGTCCCAGCGAGGTT17860.10593100961267567No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17830.10575307398622659No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17820.10569376211074356No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17750.10527857898236244No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17710.10504133148043034No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17710.10504133148043034No Hit
AGTCAGCACATTAGCAACTATGTAAATTGGTATCAGCAGATGTCAGGGAA17690.1049227077294643No Hit
ATGTAAATTGGTATCAGCAGATGTCAGGGAAAGCCCCCAAACTCCTGATC17570.10421096522366805No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17280.10249092083466044No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17210.10207573770627928No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATATCGGAACATACTACTGTCAACACTATGATAGCCTCCCCTGGACGTT17200.10201642583079627No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17130.10160124270241512No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17120.1015419308269321No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16920.10035569331727166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1251.4733814E-1049.6348470
CTAACGC553.2068783E-949.0060043
CGTATGC2350.043.9878341
TCGTATG2450.042.08899340
CCTAACG651.4037141E-841.4678462
CGAGACG2900.038.36036723
ATCTCGT2700.035.41425737
CCGTCTT2700.033.6277547
ATGCCGT2900.033.4740844
GGAGCTA3550.032.37944429
CTTATAC3800.031.922171
ACGAGAC3500.031.7805322
GTATCAA44050.031.5155451
ATCTCCG3450.031.24464410
GTACTAA553.7408303E-430.6323851
TATCAAC45500.029.990142
TCAACGC46350.029.51114
TTAACGG1501.8189894E-1229.4942335
ATCAACG46300.029.4710753
CGGTACC1650.029.46878245
Produced by FastQC (version 0.11.5)