FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686003
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72000.4270455034777518No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51930.3080065693833285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51490.3053968468620756No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49200.29181442737646374No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG40430.23979791257785427No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40200.23843373944174479No Hit
GTTCAGTGGAGGTGGATCTGGGACACATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATCGGAACATA38820.23024870062508784No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38730.22971489374574067No Hit
CATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATCGGAACATACTACTGTCAACACTATGATAGCCTC36640.21731871176978926No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35790.21227720235373246No Hit
CTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35610.21120958859503808No Hit
GAGTAAAATGTGTCCCAGATCCACCTCCACTGAACCTCGCTGGGACCCCT35020.20771018794153986No Hit
ATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA34680.2056935841751171No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34430.2042107872880416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33750.20017757975519615No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31910.1892641946663203No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGCACATTAGCAA31080.18434130900122953No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG30530.18107915584966336No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC30520.18101984397418036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30470.18072328459676523No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGCACATTAGCAA30300.1797149827135539No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30270.17953704708710483No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30150.17882530458130858No Hit
GTCCAGGGGAGGCTATCATAGTGTTGACAGTAGTATGTTCCGATATCTTC29110.17265686953107437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28850.17111476076851584No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGC28750.1705216420136856No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28280.16773398386598365No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27700.1642938950879684No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27000.16014206380415694No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26500.15717647003000587No Hit
CCACTGAACCTCGCTGGGACCCCTGTTTCCAAATTGGCAGCATCGTAGAT25990.1541515643803718No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25980.15409225250488878No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25080.14875418371141688No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24150.14323817929149593No Hit
ATACTACTGTCAACACTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGA24060.14270437241214873No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC23680.14045052114379394No Hit
GTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATGTGTCCCAGATCCACCTCCA23510.1394422192605826No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23250.13790011049802403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23060.13677318486384663No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT22870.13564625922966922No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21920.13001163105878222No Hit
ACATACTACTGTCAACACTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21870.12971507168136712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21250.1260377354014198No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21180.12562255227303867No Hit
GGCTATCATAGTGTTGACAGTAGTATGTTCCGATATCTTCAGGCTGCAGG21060.1249108097672424No Hit
CATTAGCAACTATGTAAATTGGTATCAGCAGATGTCAGGGAAAGCCCCCA20670.12259764662340458No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT20430.12117416161181209No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20410.12105553786084604No Hit
CAGTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATGTGTCCCAGATCCACCTCCA19820.1175561372073478No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19700.11684439470155154No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19660.11660714719961945No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19430.11524297406350996No Hit
CTATCATAGTGTTGACAGTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATGTGTC19360.1148277909351288No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19050.11298912279515518No Hit
CTCCTGATCTACGATGCTGCCAATTTGGAAACAGGGGTCCCAGCGAGGTT18930.11227738028935892No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18850.11180288528549476No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18760.11126907840614757No Hit
CACTATGATAGCCTCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18570.11014215277197015No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18480.10960834589262296No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT18340.10877797963586068No Hit
ATCATAGTGTTGACAGTAGTATGTTCCGATATCTTCAGGCTGCAGGCTGC18170.10776967775264933No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17530.10397371772173597No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17230.10219436145724532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAC350.001027138141.6691761
CGATTGC456.869545E-540.86773763
GTGGTAT3600.037.4033621
GTATCAA42200.035.737051
TATCAAC44850.033.159412
ATCAACG45000.033.1177523
TCAACGC44750.033.0030444
CAACGCA45500.032.4600035
AACGCAG46000.032.253456
ACGCAGA48650.030.4965827
CGCAGAG48850.030.4405948
AGAGTAC49950.029.97140111
CTAACGC450.005229952729.9031623
GCAGAGT51300.029.1179649
CAGAGTA51100.029.10025410
TGTTAGA24950.027.26030270
AATAGTC2450.026.4594336
TAACGGC1351.0617441E-825.2731436
TACGTTG700.001261369324.88100846
TGTGCAG5750.024.64291870
Produced by FastQC (version 0.11.5)