FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585473
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT65600.41375665180044063No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63120.39811463203725317No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC53250.33586191628618084No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA52890.3335913005141053No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG45110.28452077077313837No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44380.27991646656865177No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43640.2752490897038297No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG42400.267428079822236No Hit
GCCTTGGGCTGACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCCT42240.26641891725686906No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTAGAAGCAACAGTGACGTC41850.2639590835037872No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC39720.25052460685233996No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38840.24497421274282186No Hit
ACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCCTGAGAGAGTTGA35850.22611548730252737No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGTACTTCAAAT34830.2196820759483132No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29930.18877647238395104No Hit
GTCGATCACCATCTCCTGCACTAGAAGCAACAGTGACGTCCGAAATTATAATTTTGTCTCCTGGTACCAACAACA29690.18726272853590065No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29270.18461367680181245No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28500.1797570819559841No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28250.1781802654475983No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCCTGGTC28210.17792797480625655No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27780.17521585041183294No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25580.16133986513803766No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25560.16121371981736682No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24970.15749243285757628No Hit
GACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCCTGAGAGAGTTG23890.1506805855413495No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23310.14702237124189438No Hit
GGCAAAGGCCCCCAACTCCTGATTTATGAGGGCAATAAGCGGCCCTCAGG23240.14658086261954634No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22870.14424717418713534No Hit
CTCCTGCACTAGAAGCAACAGTGACGTCCGAAATTATAATTTTGTCTCCTGGTACCAACAACACACAGGCAAAGG22840.14405795620612902No Hit
GATTATTATTGTACTTCAAATACAAGCATCAACTCTCTCAGGGTCTTCGG22540.14216577639606603No Hit
CTCTCAGGGTCTTCGGAACTGGGACCAGGGTCACCGTCCTGGGTCAGCCC21600.13623694632453534No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTAGAAGCAACAGTGACGT20940.13207415074239676No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20680.1304342615736755No Hit
GCCCAGAGATGGTCAGGGAGGCCATGTTGCCAGACTTGGAGCCAGAGAAG20170.1272175558965684No Hit
CCCTGAGAGAGTTGATGCTTGTATTTGAAGTACAATAATAATCAGCCTCG19550.12330705095577156No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19330.12191945242839204No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18980.11971190931665188No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18770.1183873834496078No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18380.11592754969652588No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18200.1147922418104881No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG17990.113467715943444No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17830.11245855337807709No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17810.11233240805740621No Hit
CCCCTGAGGGCCGCTTATTGCCCTCATAAATCAGGAGTTGGGGGCCTTTGCCTGTGTGTTGTTGGTACCAGGAGA17490.11031408292667234No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17380.10962028366298258No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17300.10911570238029912No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17200.1084849757769448No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16750.10564670606185032No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16710.10539441542050858No Hit
CAATAATAGAGGGCCGAGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA16680.10520519743950228No Hit
CTATTATTGTGCGAGAGATGGATATAACTTCGCTATGGACGTCTGGGGCC16620.10482676147748969No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16560.10444832551547709No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16500.10406988955346448No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16320.1029345816674267No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16100.10154698314004716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT252.610765E-455.01345439
TCGTATG1550.046.66609240
ACTAGCG2850.045.25521527
GGTATCA14100.044.9591141
TAGCGCT2800.044.90646729
CTAGCGC2800.044.87240628
GACTAGC3000.042.97480826
CTAACGC405.5508914E-542.3718953
GAGACTA3150.041.97232424
CGTATGC1550.040.06500241
TATGCGG458.4261104E-539.47568556
ACGAGAC3500.038.7375722
CACGAGA3500.038.7351321
CGAGACT3650.037.15030323
GTGGTAT3750.037.0636181
GTATCAA37650.037.0059931
AGACTAG3500.036.8169525
CTTATAC3700.036.6482771
CGCTCAT2850.035.86941532
GCGCTCA3450.035.53046831
Produced by FastQC (version 0.11.5)