FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585473
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT58440.36859662700027057No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53780.33920476728395876No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC52240.3294915775923021No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48550.30621776592852734No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC46720.29467546908714304No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC46640.2941708878044596No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43440.27398763649712105No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42880.27045556751833677No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG41740.26326528424009743No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTAGAAGCAACAGTGACGTC38640.24371275953611318No Hit
GCCTTGGGCTGACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCCT36690.23141359077070378No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35830.22598934198185652No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGTACTTCAAAT33990.21438397248013685No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33000.20813977910692896No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30760.19401150319179197No Hit
ACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCCTGAGAGAGTTGA30680.1935069219091085No Hit
GTCGATCACCATCTCCTGCACTAGAAGCAACAGTGACGTCCGAAATTATAATTTTGTCTCCTGGTACCAACAACA29800.1879565277995904No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29110.18360451423644553No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28300.17849562874927546No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28140.1774864661839085No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28130.1774233935235731No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27990.17654037627887703No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCCTGGTC27530.1736390339034471No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25650.1617813737603857No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24180.15250969269107706No Hit
CTCCTGCACTAGAAGCAACAGTGACGTCCGAAATTATAATTTTGTCTCCTGGTACCAACAACACACAGGCAAAGG22950.1447517554698188No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC22820.14393181088545814No Hit
GATTATTATTGTACTTCAAATACAAGCATCAACTCTCTCAGGGTCTTCGG22410.1413458318117054No Hit
GGCAAAGGCCCCCAACTCCTGATTTATGAGGGCAATAAGCGGCCCTCAGG21890.13806605347426287No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21210.13377711257145344No Hit
CTCTCAGGGTCTTCGGAACTGGGACCAGGGTCACCGTCCTGGGTCAGCCC20990.13238951404407392No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20910.13188493276139046No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20910.13188493276139046No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTAGAAGCAACAGTGACGT20450.12898359038596052No Hit
GCCCAGAGATGGTCAGGGAGGCCATGTTGCCAGACTTGGAGCCAGAGAAG19470.1228024696730881No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19000.11983805463732275No Hit
CCCTGAGAGAGTTGATGCTTGTATTTGAAGTACAATAATAATCAGCCTCG18350.11573833171551959No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18300.11542296841384245No Hit
GACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCCTGAGAGAGTTG18230.1149814597914944No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18130.11435073318814006No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18120.11428766052780463No Hit
CCCCTGAGGGCCGCTTATTGCCCTCATAAATCAGGAGTTGGGGGCCTTTGCCTGTGTGTTGTTGGTACCAGGAGA18020.1136569339244503No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18000.11353078860377945No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17770.11208011741606447No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17160.10823268513560305No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16990.1071604499099007No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16960.1069712319288944No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16760.10570977872218575No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG16490.10400681689312904No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16480.10394374423279362No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16280.10268229102608498No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16240.10243000038474323No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGTACTT15970.10072703855568653No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15870.1000963119523322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1152.730303E-949.89505870
GGTATCA15900.043.6656071
GTGGTAT4150.038.355931
GTATCAA40750.036.9011
TATCAAC45250.032.6211972
ATCAACG45600.032.2131773
TCAACGC46000.031.8564724
AACGCAG46700.031.3849036
CAACGCA46850.031.355685
TGGTATC5300.030.661782
CGCAGAG50200.029.7361438
ACGCAGA49950.029.4783767
AGAGTAC50700.029.1090711
CAGAGTA51450.028.8821110
GCAGAGT51550.028.7604229
AATGGTA8500.028.6896670
AATAGTC2400.028.5045236
CGACAAA650.00725279827.96642969
GTACGTA1304.471476E-727.96642969
GTCGTCC500.007412062527.84278146
Produced by FastQC (version 0.11.5)