FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922676
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAAAGGACGTTATAGTGTGACCTACTACTTTGACTA23510.2548023358145221No Hit
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATACA20390.2209876489688688No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18140.19660205749363807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15080.16343765308732425No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15020.16278737064798476No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14720.15953595845128735No Hit
GTATAACAGTGCCCCTCAGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14410.15617616584803332No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13320.144362701533366No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13280.14392917990713966No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12620.1367760730744053No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12310.1334162804711513No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12100.1311402919334631No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11620.1259380324187472No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11460.1242039459138419No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10870.11780950192700362No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9970.10805526533691134No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9820.10642955923856261No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9740.10556251598610998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9660.10469547273365733No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA9660.10469547273365733No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9560.10361166866809152No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9460.1025278646025257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT253.1556701E-668.7706539
ATCGCGT253.210731E-668.5706337
AATCGCG309.545545E-657.063936
TTTCGCC200.00791736750.800722
CGTATGC2350.049.96347441
TCGTATG2000.048.23769440
ATGCCGT2450.048.15038344
ATCTCGT1750.043.1015437
TCGCGTA405.1469768E-542.91552438
GTATGCC2850.042.48010342
TCTCCGA4450.041.84464311
GCGTATG503.876139E-641.34659240
ACGAGAC4200.041.12437422
CACGAGA4200.041.11991521
CCGTCTT2550.041.06584547
CTTATAC4500.040.631751
TATACAC5000.040.6295473
ATCTCCG4500.040.62734610
TCCGAGC4450.040.3230213
TTATACA4900.040.0767632
Produced by FastQC (version 0.11.5)