FastQCFastQC Report
Wed 8 May 2024
EGAF00004692957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032778
Total Bases76.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42560.4120924341920529No Hit
CCTCTGAGGAGCTCCAAGCCACCAAGGCCACACTACTGTGTCTGATCAGT41090.39785897840581425No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC40020.38749857181310987No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC33930.32853139784154967No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGAGCTG33830.3275631355431661No Hit
CAGTAGTGTGGCCTTGGTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA33250.3219472142125413No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT32510.31478207320450285No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32430.31400746336579594No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC32000.3098439354827465No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31880.3086820207246862No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCTCTCTTTATGTCTTCGG30710.2973533518335983No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC30280.29318982395054893No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT29660.28718659770057064No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAGAGAGCTGC28460.2755674501199677No Hit
CTACTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG28290.2739214042127156No Hit
ACACTACTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27660.26782135173289906No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAGCTATGTCTCCT27020.2616244730232441No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT25090.24293701066444096No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA24870.2408068336079971No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA23580.22831624995884886No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23270.22531463683385977No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA23260.2252178106040214No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT22500.21785901713630615No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC22500.21785901713630615No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21740.21050022366859092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21710.21020974497907588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA21570.20885417776133883No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20910.2024636465920072No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC20340.19694455149122078No Hit
CTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAA19010.18406666292271912No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCACCAAGGCCACACTACTGT18810.18213013832595193No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT17790.1722538628824394No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG17760.17196338419292434No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17660.17099512189454075No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17380.1682839874590667No Hit
GTAGTGTGGCCTTGGTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGA17320.16770303008003656No Hit
GACATAAAGAGAGCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCT17070.1652823743340776No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16980.16441093826553238No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16830.16295854481795702No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16100.15589023003975683No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCACCAAGGCC15990.1548251415115349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.15405053167282806No Hit
GTGTATTACTGTGCGAGAACGTTTTACTATGATAGTAGTGGTAATTCACC15880.15376005298331297No Hit
GGTTATAGCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA14930.14456156114866894No Hit
ACACAGTAGTGTGGCCTTGGTGGCTTGGAGCTCCTCAGAGGAGGGCGGGA14800.14330282016077028No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14650.1418504267131949No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT14500.14039803326561953No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14160.13710594145111535No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14120.13671863653176192No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG13870.13429798078580296No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG13810.1337170234067728No Hit
CATAAAGAGAGCTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCG13680.13245828241887414No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC13610.13178049881000564No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13580.13149002012049055No Hit
GACACAGTAGTGTGGCCTTGGTGGCTTGGAGCTCCTCAGAGGAGGGCGGG13360.1293598430640467No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG13120.12703601354792607No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13050.12635822993905757No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGG12830.12422805288261368No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAGTAGTGTGGCC12420.120258177459241No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCA12360.11967722008021085No Hit
CTCTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC12270.11880578401166564No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12270.11880578401166564No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT12120.11735339056409026No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA12100.11715973810441355No Hit
GATCAGACACAGTAGTGTGGCCTTGGTGGCTTGGAGCTCCTCAGAGGAGG12000.11619147580602994No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11940.1156105184269998No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11660.11289938399152576No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11410.11047872824556682No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC11390.11028507578589009No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11140.10786442003993114No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10960.10612154790284069No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCC10930.10583106921332561No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10870.10525011183429546No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10690.10350723969720502No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTG10690.10350723969720502No Hit
AGTGTGGCCTTGGTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT10520.10186119378995293No Hit
GGCCACACTACTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG10490.10157071510043786No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCT10450.10118341018108441No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA10370.10040880034237756No Hit
CCACACTACTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTG10330.10002149542302412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)