FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452111
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCGTCTCTCAGACTGCTCATTTGCAGATACA47750.32883161135753397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34630.23848039165050056No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33910.2335220930080414No Hit
GTCTATTACTGTGCGAGAGGTGGGGCTGGGGATCATGGATACTGGGGCCA31140.2144464162863583No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28640.19723010155559734No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGGCTGGAGCCTGAAGATT27220.18745123478852513No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA25680.17684598491437636No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24960.17188768627191725No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24300.16734257918299633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23950.16493229512068983No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23730.16341725942438284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23240.1600428617371537No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23080.158941017594385No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTTTCAACTATGCCATGTGCTGGGTCCGCCGGGCTCCAGGGAAGGGG22650.15597981146069412No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20970.14441044796162278No Hit
GAGTATTAGCAGCTACTCCTTCGCCTGGTACCAGCAGAAACCTGGCCAGG20920.14406612166700755No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20490.14110491553331667No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20230.13931441880131754No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20190.13903895776562536No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20170.13890122724777929No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18670.1285714384093227No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAATGGCCCTACT18280.125885693311324No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18200.12533477123993966No Hit
GTTTATTACTGTCAGCAGAATGGTAGTCCGCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18130.12485271442747835No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17910.1233376787311714No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17750.12223583458840268No Hit
ACCTAGACCTCTGTACGCAGACTCCGTGAAGGGCCGGTTCACCATTTCCA17430.12003214630286528No Hit
GAATGGTAGTCCGCCATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17370.11961895474932702No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17000.1170709401691744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16970.11686434439240527No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16680.114867251883637No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG16590.1142474645533296No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16300.11225037204456133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16300.11225037204456133No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTTTCAACTATGCCATGTGCTGGGTCCG16050.11052874057148523No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15970.1099778185001009No Hit
GTCTGAGAGACGAGGACACGGCCGTCTATTACTGTGCGAGAGGTGGGGCT15690.10804959125025566No Hit
GTAATAAACCGCAGAATCTTCAGGCTCCAGCCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA15620.10756753443779436No Hit
GACTTCACTCTCACCATCAACAGGCTGGAGCCTGAAGATTCTGCGGTTTATTACTGTCAGCAGAATGGTAGTCCG15500.10674115133071783No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15370.10584590296471826No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15320.10550157667010304No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15290.10529498089333392No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15230.10488178933979565No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15100.10398654097379609No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14960.10302242734887346No Hit
CTCCCAGACTCCTCGTCTCTGGTACATCTAGTAGGGCCATTGGCATCCCA14930.10281583157210435No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14920.1027469663131813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14910.10267810105425824No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14820.10205831372395087No Hit
CTACCATTCTGCTGACAGTAATAAACCGCAGAATCTTCAGGCTCCAGCCTGTTGATGGTGAGAGTGAAGTCTGTC14690.10116306535795129No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14680.10109420009902824No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14660.10095646958118215No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14540.10013008647410562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)