FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503755
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTCCAGACTGACGATGAGGCTGATTATTATTGTTGTTCATTT38080.25323274070576657No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37810.2514372354539137No Hit
GCGTTGGGCTGACCGACGACGGTCACCTTGGTCCCTCCGCCGAGGATCCT35990.23933420005253517No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33610.22350715375842475No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCGTTGGGCTGACCGACGACGGTCACCTTGGTCCCTC30220.20096358781849435No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28690.19078905805799481No Hit
GTGTCCGGGTCTCCTGGCCAATCAGTCACCATCTCCTGCACTGGAACCAG27860.1852695419134101No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG26300.17489551156937136No Hit
GTAATAAACAGCCGTGTCCGCGGCGGAAACAGAATTCAGGACCAGGGAGA25560.16997449717540425No Hit
GTGTATGGTGGGTCCTTCAGTGGTCAATACTGGGCCTGGCTCCGCCAGTC24780.16478748200338486No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG24090.1601989685819831No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGAGCTTATAACTATGTCTCTTGGTACCAGCATCACCCAGGCAGAGC23610.1570069592453558No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22250.14796293279157843No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21640.1439064209262812No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21610.14370692034274202No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21350.1419779152854022No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCGTTGGG21010.13971690867195788No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20970.1394509078939056No Hit
GTATAATCTCGATAAGCGACCCTCAGGGGTCCCCAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC20390.1355938966121476No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20360.13539439602860837No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20300.13499539486152998No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19730.13120488377428505No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG19140.12728137229801398No Hit
AGATTATACATGATGAGCTTGGGGGCTCTGCCTGGGTGATGCTGGTACCA18520.12315836023820369No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18520.12315836023820369No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18500.12302535984917756No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18470.12282585926563835No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAACCTCGCTCAGTGTCCGGGTCTCCTGGCCAATCAGTCACCATCTCCTG18330.12189485654245538No Hit
CCTTGGTCCCTCCGCCGAGGATCCTATTGAGATTAGCAAATGAACAACAATAATAATCAGCCTCATCGTCAGT18210.12109685420829856No Hit
GACTCAACCTCGCTCAGTGTCCGGGTCTCCTGGCCAATCAGTCACCATCT17740.11797134506618431No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17500.11637534039787066No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17370.11551083786920076No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAACCTCGCTCAGTGTCCGGGTCTCCTGGCCAATCAGTCAC17220.11451333495150473No Hit
CCCAGGCAGAGCCCCCAAGCTCATCATGTATAATCTCGATAAGCGACCCTCAGGGGTCCCCAATCGCTTCTCTGG16980.11291733028319108No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16960.11278432989416494No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16790.11165382658744277No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16720.11118832522585129No Hit
GTTTATTACTGTGCGAGGAGTCGTTGGGAAAATTACTATCCCACTTACGC16700.11105532483682515No Hit
GGAACAGAGTGACCGAGGGGGCAGCGTTGGGCTGACCGACGACGGTCACC16680.11092232444779901No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16580.11025732250266831No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16550.1100578219191291No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15970.10620081063737112No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15940.1060013100538319No Hit
CCCCTGAGGGTCGCTTATCGAGATTATACATGATGAGCTTGGGGGCTCTGCCTGGGTGATGCTGGTACCAAGAGA15350.10207779857756083No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15280.10161229721596936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAT6400.056.77769570
TTCGATA200.007094760452.2315845
GGTATCA17850.044.065381
TCGTATG2450.042.1198240
ATCTCGT2250.040.9927337
CTAGCGT350.001532560738.412144
CGTATGC2550.037.86341
GTATCAA48150.037.6281051
CCCACGA4200.036.001819
TCTCCGA3950.035.7224311
ACGCTTT5250.035.33411847
CCACCGA7450.034.8570569
AGGCAAT3700.034.75951832
CTTACGC4900.034.73486744
ACTTACG5000.034.6514843
TACGCTT5100.034.2243946
GGCAATC3300.033.89702233
CGTCTTC2900.033.87011748
CGAGACA4500.033.61509323
CCTAACG502.1699737E-433.611742
Produced by FastQC (version 0.11.5)