FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503755
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37810.2514372354539137No Hit
TCTCTGGACTCCAGACTGACGATGAGGCTGATTATTATTGTTGTTCATTT37680.2505727329252438No Hit
GCGTTGGGCTGACCGACGACGGTCACCTTGGTCCCTCCGCCGAGGATCCT36800.24472071580809376No Hit
GCCCAACGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35350.23507818760369875No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34720.2308886753493754No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33480.22264265122975488No Hit
GTGTCCGGGTCTCCTGGCCAATCAGTCACCATCTCCTGCACTGGAACCAG29050.19318306506046531No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCGTTGGGCTGACCGACGACGGTCACCTTGGTCCCTC27910.18560204288597545No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27250.18121303004811287No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26300.17489551156937136No Hit
GTAATAAACAGCCGTGTCCGCGGCGGAAACAGAATTCAGGACCAGGGAGA25350.1685779930906298No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG25220.1677134905619599No Hit
GTGTATGGTGGGTCCTTCAGTGGTCAATACTGGGCCTGGCTCCGCCAGTC25090.16684898803328999No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGAGCTTATAACTATGTCTCTTGGTACCAGCATCACCCAGGCAGAGC24590.16352397830763654No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23620.15707345943986886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22010.14636692812326477No Hit
GTATAATCTCGATAAGCGACCCTCAGGGGTCCCCAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC21900.145635425983621No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21830.14516992462202952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20040.13326638980419017No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG19690.13093888299623277No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19370.12881087677181458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18610.12375686198882131No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAACCTCGCTCAGTGTCCGGGTCTCCTGGCCAATCAGTCAC18580.12355736140528212No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18540.12329136062722984No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18370.12216085732050766No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCGTTGGG18350.12202785693148153No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18240.12129635479183776No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAACCTCGCTCAGTGTCCGGGTCTCCTGGCCAATCAGTCACCATCTCCTG17900.11903534817839342No Hit
CCTTGGTCCCTCCGCCGAGGATCCTATTGAGATTAGCAAATGAACAACAATAATAATCAGCCTCATCGTCAGT17650.11737284331556669No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17520.11650834078689681No Hit
GTTTATTACTGTGCGAGGAGTCGTTGGGAAAATTACTATCCCACTTACGC17450.11604283942530531No Hit
GACTCAACCTCGCTCAGTGTCCGGGTCTCCTGGCCAATCAGTCACCATCT17110.11378183281186098No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17100.1137153326173479No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16920.11251832911611265No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16800.11172032678195584No Hit
AGATTATACATGATGAGCTTGGGGGCTCTGCCTGGGTGATGCTGGTACCA16380.10892731861240695No Hit
GGAACAGAGTGACCGAGGGGGCAGCGTTGGGCTGACCGACGACGGTCACC16270.10819581647276318No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16220.10786331550019783No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAAC15920.10586830966480577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15890.10566880908126657No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15810.10513680752516201No Hit
CCCAGGCAGAGCCCCCAAGCTCATCATGTATAATCTCGATAAGCGACCCTCAGGGGTCCCCAATCGCTTCTCTGG15470.10287580091171766No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15430.10260980013366539No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15380.10227729916110005No Hit
CCCCTGAGGGTCGCTTATCGAGATTATACATGATGAGCTTGGGGGCTCTGCCTGGGTGATGCTGGTACCAAGAGA15060.10014929293668184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)