FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543224
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGCC80870.5240328040517773No Hit
GCTTAATACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT69510.45042067774995725No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50570.32769060097561986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50150.32496902588347515No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35570.23049149054187854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35000.2267979243453964No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG34870.22595553205497065No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34380.22278036111413507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34010.22038278305676948No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT33810.2190867949176529No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGATTTCA33650.21805000440635966No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28660.18571510033540173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28500.1846783098241085No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAATGATTTAGCCTGGTATCAGCAAGAA28200.18273432761543365No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28010.18150313888327294No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27940.18104954303458215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27720.17962395608155393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27370.17735597683809998No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27290.17683758158245336No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTCTTGCTGATACCAGGC27040.17521759640855766No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27020.175087997594646No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26870.1741160064903086No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25240.16355370315650872No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTG23910.15493538203138366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23780.1540929897409579No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT23530.1524730045670622No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23520.15240820516010636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23460.1520194087183714No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23210.15039942354447572No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23040.14929783362622664No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG22920.14852024074275674No Hit
GATTTAGCCTGGTATCAGCAAGAACCAGGGAAAGCCCCTAACCTCCTGAT22630.14664105794103774No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21860.14165150360543902No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21310.13808753622286848No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20480.13270918544553478No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC19900.1289508198420968No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTCTTGCTGATACCAGGCTAAAT19860.1286916222142735No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19670.12746043348211278No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19330.12525725364561463No Hit
ATACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGA18620.12065649575175089No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18510.11994370227523678No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18430.11942530701959016No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18390.11916610939176685No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17940.11625013607875462No Hit
GGTAAGTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAAT17760.11508374675354971No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17710.11475974971877057No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17580.11391735742834481No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17510.11346376157965403No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17310.11216777344053748No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGATTTCACTCTCA17260.11184377640575834No Hit
ACTTATTACTGTCAACAGCTTAATACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAA17170.11126058174315588No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC17130.11100138411533259No Hit
GGGCATTAGCAATGATTTAGCCTGGTATCAGCAAGAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATC16850.10918700072056942No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATACTTACCCATTCACTTT16710.10827980902318782No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTCTTG16110.10439184460583817No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16070.10413264697801486No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATACTTAC15790.10231826358325169No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15770.10218866476934002No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15750.10205906595542838No Hit
GGTTAGGGGCTTTCCCTGGTTCTTGCTGATACCAGGCTAAATCATTGCTA15730.10192946714151672No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15530.10063347900240018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACT200.00698634452.43548652
GTGGTAT2600.047.3227271
TAACAGT15250.041.6461270
CGAACCA350.001128924540.87631264
TCGTATG1550.040.01162340
CGTATGC1750.037.44684641
GTATCAA25100.036.7646681
CTTATAC2050.035.011121
GTCGTAT400.002625222834.42296639
ATCTCGT1400.034.36911437
TGGACCG501.9688244E-434.1730965
ACGAGAC2050.033.34500522
GCCGTCT1900.032.85359646
ACATGCG1900.032.4007627
GACAGGT1500.031.8990211
TGTCGTA652.3709605E-531.74913438
TATCAAC29000.031.5816732
GATGTCG553.3443386E-431.2232836
ATCAACG30100.030.7671363
CGAGACA2350.030.54557623
Produced by FastQC (version 0.11.5)