FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543224
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGCC81910.5307719423751833No Hit
GCTTAATACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT62380.4042187005904522No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53120.3442144497493559No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44410.28777416629083014No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35730.23152828105317177No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33810.2190867949176529No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGATTTCA32870.2129956506638051No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32520.21072767142035118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32310.2093668838742788No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31650.20509012301519416No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31040.20113735919088868No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30150.19537021197182006No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT29840.19336143035618938No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29290.18979746297361888No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG29230.1894086665318839No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAATGATTTAGCCTGGTATCAGCAAGAA26670.17282001835119207No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26430.17126483258425218No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25400.16459049366780196No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTG25120.16277611027303876No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25000.16199851738956883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24370.1579161547513517No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24230.15700896305397014No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTCTTGCTGATACCAGGC23670.1533801962644438No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23120.14981622888187326No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23100.14968663006796162No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22790.14767784845233098No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22590.1463818603132144No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22470.14560426742974447No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG22070.14301229115151137No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT21790.1411979077567482No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21340.13828193444373596No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21130.1369211468976636No Hit
GATTTAGCCTGGTATCAGCAAGAACCAGGGAAAGCCCCTAACCTCCTGAT20900.13543076053767958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.13484756587507712No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20640.13374597595682805No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20440.13244998781771147No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20340.1318019937481532No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19910.12901561924905264No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19260.12480365779692383No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC18400.11923090879872268No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18010.1167037319274454No Hit
GGTAAGTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAAT17640.11430615387007978No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17530.11359336039356567No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTCTTGCTGATACCAGGCTAAAT17420.11288056691705158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17110.11087178530142092No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17000.11015899182490682No Hit
GAGTGAAATCTGCCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA16800.10886300368579027No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16800.10886300368579027No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16780.1087334048718786No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATACTTACCCATTCACTTT16660.10795581198840869No Hit
ATACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGA16650.10789101258145285No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGATTTCACT16550.10724301851189459No Hit
ACTTATTACTGTCAACAGCTTAATACTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAA16550.10724301851189459No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.10646542562842466No Hit
GGGCATTAGCAATGATTTAGCCTGGTATCAGCAAGAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATC16320.10575263215191054No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16320.10575263215191054No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA16090.10426224579192653No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15940.1032902546875891No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15870.10283665883889831No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGCCCCAGATCCACTGCCG15700.10173506892064924No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATACTTAC15550.10076307781631182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)