FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281610
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTCCAGATATAGGGAGCTCTTGGCGTTGTCTCTGG36100.2816769532072939No Hit
GACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAAGTTGTTGCCGCTTGCATATGAGCTGCAGTAATAAT34810.2716114886744017No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30240.23595321509663628No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCCGTGACATTGGTACTTATAACTATGTCTCCTGGT25220.19678373296088517No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23440.18289495244263076No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCCGATTATTACTGCAGCTCATAT23310.18188060330365713No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA22780.17774517989091845No Hit
CTCTATTACTGTGTAAAGTGGCCTCTGGCGTGGCTGGGCGGCTACTACTT22760.17758912617723022No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTC22290.17392186390555628No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22060.1721272461981414No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAAGTTGTTGCCGCTTGCATATGAGCTG21550.168147876499091No Hit
CTATTACTGTGTAAAGTGGCCTCTGGCGTGGCTGGGCGGCTACTACTTTGACTCCTGGGGCCAGGGAACCCAGGT20970.16362231880213168No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20880.16292007709053455No Hit
CAGTAATAGAGGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTCCAGATA20480.15979900281676954No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG20150.1572241165409134No Hit
CTCCAGGCCCTTCCCTGGAACTTGTCGGACCCACGACATGGCATAATCATCAAAGGAGAATCCAGAGGCTGCAC19760.15418106912399246No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19440.15168420970498045No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19240.15012367256809794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19160.1494994577133449No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18790.14661246401011227No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18760.1463783834395799No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18760.1463783834395799No Hit
CTCCTGCACTGGAACCGGCCGTGACATTGGTACTTATAACTATGTCTCCTGGTATCAACAACGACCAGGCAAAGC18540.14466179258900916No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT18100.14122861088786762No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17920.13982412746467335No Hit
GAGTTGGGACTGACCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCATCTGGTGGAGT17500.13654699947722007No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG17440.1360788383361553No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC17020.13280171034870203No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16770.13085103892759886No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16590.12944655550440462No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16390.12788601836752211No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16350.12757391094014559No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCTTTGATGATTATGCC16140.12593534694641895No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15990.12476494409375707No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15830.12351651438425107No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGACCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCC15770.1230483532431863No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15770.1230483532431863No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15470.12070754753786253No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15450.12055149382417428No Hit
CTCCTGTGCAGCCTCTGGATTCTCCTTTGATGATTATGCCATGTCGTGGGTCCGACAAGTTCCAGGGAAGGGC15400.12016135953995366No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15380.1200053058262654No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC15100.11782055383462989No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAG14980.11688423155250037No Hit
GTCAGTCACCATCTCCTGCACTGGAACCGGCCGTGACATTGGTACTTATAACTATGTCTCCTGGTATCAACAACG14930.11649409726827974No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC14710.11477750641770898No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14410.1124367007123852No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14410.1124367007123852No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG14380.11220262014185282No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14350.11196853957132044No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG14350.11196853957132044No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA14300.11157840528709982No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14290.1115003784302557No Hit
CTCCAGAGACAACGCCAAGAGCTCCCTATATCTGGAAATGAACAGTCTGAGAGCTGAGGACACGGCCCTCTATT14100.11001786815021732No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14100.11001786815021732No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG13950.10884746529755542No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13900.1084573310133348No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13650.10650665959223164No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13490.10525822988272564No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA13460.10502414931219325No Hit
GTTTGGTCCCTCCGCCGAAAACCAAGTTGTTGCCGCTTGCATATGAGCTG13310.10385374645953138No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13300.10377571960268725No Hit
GGCCTGGAGTGGGTCTCAAGTATTGATTGGAATAGTCATTCCACACGCTATGCGGAATTTGTGAAGGGCCGATTC13200.102995451034246No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13050.1018250481815841No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13000.10143491389736348No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGT12920.10081069904261046No Hit
GTCTGAGAGCTGAGGACACGGCCCTCTATTACTGTGTAAAGTGGCCTCTGGCGTGGCTGGGCGGCTACTACTTTG12910.10073267218576636No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12900.10065464532892222No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12890.10057661847207809No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12880.10049859161523397No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12870.10042056475838984No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12850.10026451104470158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3150.070.9755670
CGCGTAT208.868748E-568.4343439
GTGGTAT2200.052.381771
CGTATGC900.049.53014841
GTAGTTT1302.729245E-547.77202270
TCGTATG900.045.68587540
CTACGCT1450.044.47888628
ACTACGC1450.044.46325327
ATCTCGT950.043.12825837
ACGAGAC1500.042.93586322
TACGCTG1550.041.62880729
AGAGTCG1200.039.5415611
CGAGACT1750.036.80360423
TCTCGTA1051.8189894E-1135.80002638
CTTATAC1800.035.7770921
ACCCAGG17900.035.38865770
CACGAGA1900.033.89673621
GACTACG1950.033.0482226
TTTTCGG18850.032.9462270
TACACAT2300.032.41795
Produced by FastQC (version 0.11.5)