FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281610
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAAGTTGTTGCCGCTTGCATATGAGCTGCAGTAATAAT34250.2672419846911307No Hit
GTAATAGAGGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTCCAGATATAGGGAGCTCTTGGCGTTGTCTCTGG30940.24141509507572506No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27020.21082856719282778No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCCGTGACATTGGTACTTATAACTATGTCTCCTGGT25020.19522319582400263No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA23750.18531378500479867No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23110.1803200661667746No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAAGTTGTTGCCGCTTGCATATGAGCTG21810.17017657477703826No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCCGATTATTACTGCAGCTCATAT21800.17009854792019413No Hit
CTCTATTACTGTGTAAAGTGGCCTCTGGCGTGGCTGGGCGGCTACTACTT21580.16838195706962336No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTTTGGTCCCTC21280.1660411513642996No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21130.16487074851163772No Hit
CTATTACTGTGTAAAGTGGCCTCTGGCGTGGCTGGGCGGCTACTACTTTGACTCCTGGGGCCAGGGAACCCAGGT19480.15199631713235695No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT18620.14528600744376216No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18480.1441936314479444No Hit
CAGTAATAGAGGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTCCAGATA18370.143335336022659No Hit
GAGTTGGGACTGACCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCATCTGGTGGAGT18000.14044834231942635No Hit
CTCCTGCACTGGAACCGGCCGTGACATTGGTACTTATAACTATGTCTCCTGGTATCAACAACGACCAGGCAAAGC17780.13873175146885558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17580.13717121433197307No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17510.13662502633406418No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17390.13568870405193467No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG17290.1349084354834934No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC17250.1345963280561169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16940.132177495493949No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16830.13131920006866366No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16830.13131920006866366No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16750.13069498521391062No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16750.13069498521391062No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16600.12952458236124875No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16340.12749588408330145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16260.12687166922854845No Hit
CTCCTGTGCAGCCTCTGGATTCTCCTTTGATGATTATGCCATGTCGTGGGTCCGACAAGTTCCAGGGAAGGGC16020.12499902466428946No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15610.1217999235336803No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15460.1206295206810184No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15410.12023938639679778No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15350.11977122525573301No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGACCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCC15170.11836674183253876No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCTTTGATGATTATGCC14990.1169622584093445No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14990.1169622584093445No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14960.11672817783881212No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14630.11415329156295596No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14580.11376315727873534No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14200.11079813671865857No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG14150.11040800243443794No Hit
GTCAGTCACCATCTCCTGCACTGGAACCGGCCGTGACATTGGTACTTATAACTATGTCTCCTGGTATCAACAACG14090.10993984129337318No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC13710.10697482073329641No Hit
CTCCAGGCCCTTCCCTGGAACTTGTCGGACCCACGACATGGCATAATCATCAAAGGAGAATCCAGAGGCTGCAC13540.10564836416694626No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13310.10385374645953138No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG13270.10354163903215487No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13200.102995451034246No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13090.10213715560896061No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12910.10073267218576636No Hit
GTTTGGTCCCTCCGCCGAAAACCAAGTTGTTGCCGCTTGCATATGAGCTG12860.10034253790154571No Hit
GTCTGAGAGCTGAGGACACGGCCCTCTATTACTGTGTAAAGTGGCCTCTGGCGTGGCTGGGCGGCTACTACTTTG12830.10010845733101333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTG200.007477964751.5408743
GTGGTAT3700.051.288021
TTTTCGG18600.044.14200270
CGGACCG508.329886E-443.4786269
TCACATG2901.4824764E-939.2009170
GGTACGA451.1183156E-437.622522
GTAGTTT1350.001739459637.4263870
TGTCAGT1400.002079494536.08972570
AAGCGGT12950.036.08972570
TTTAAGT3203.8817234E-935.5258270
TATCTTG1450.002470187434.84525370
GCACCGT12050.034.59222870
TCTCGCG8400.034.58598770
ACCCAGG17050.033.33801770
TCTTCGG2701.4591569E-632.7480870
GAGCCAT16700.032.52397570
ACTGGTG25750.031.88509870
AATACGT751.939119E-631.5881210
ATCAACG28900.031.4997833
GTGGCCG14750.030.82918770
Produced by FastQC (version 0.11.5)