FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629838
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23760.3772398616787174No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCC10200.16194640526611606No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10190.16178763428056103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.14559299375394943No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCA7850.12463522366068734No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6910.10971075101851588No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGAC6800.1079642701774107No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC6370.10113711779854502No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGG6350.10081957582743499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTCG11700.054.55264370
AGGGGGG1851.8553692E-952.07677570
ACCGCAT700.007426817451.611870
GTGGTAT3500.048.9426771
TGGTATC3650.046.923882
GGTATCA12950.044.8706051
TTATGCG504.6236837E-640.296134
GTATCAA32150.039.3863681
TAGGTTT1350.00219574835.6822370
TCGTCCG1450.00311717333.2213970
TCGTTGC450.003666218832.15154362
TCGTATG751.7833172E-631.92441640
CTCGTAT751.8128048E-631.85742839
CGGTCTA652.3876493E-531.7120942
TATCAAC41400.030.8247722
TATGCGG553.8141856E-430.5273745
CGTATGC902.101824E-730.48062141
TACACGG1200.001768390330.10688270
TAATACG450.00525768329.8679624
ATCAACG42850.029.703323
Produced by FastQC (version 0.11.5)