FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744433
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGAAGCAAACA42270.5678146992408988No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24390.3276319024008877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22530.3026464436692087No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21920.2944522878486042No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATAAGGGATCACTATCACTATCCCACACCTGACAGT20460.2748400460484691No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC19960.26812352488403923No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19540.2624816471059182No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18310.24595900504142076No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17940.2409887793797427No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17150.23037667593994357No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATAAGGGATCACTATCACTATCCCACACCTGACAGTA16870.22661542408786284No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT16020.21519733810833214No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT15820.21251072964256018No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15690.21076443413980844No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC15490.2080778256740365No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGAAGCAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGA15040.20203295662604964No Hit
GGATTACCTGTGAAGCAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC14150.1900775489533645No Hit
GTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG13840.18591330583141802No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCC13180.17704749789437063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12940.1738235677354443No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT12450.16724137699430305No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12120.16280847302577936No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12090.16240548175591357No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC11870.15945021244356444No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC11460.15394266508873194No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT11390.15300235212571178No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG11390.15300235212571178No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATAAGGGAT11140.14964409154349687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11000.1477634656174565No Hit
GTGATAGTGATCCCTTATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCC10930.14682315265443632No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTGAT10710.1438678833420872No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACT10690.14359922249551No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG10630.14279323995577842No Hit
CATATAAGGGATCACTATCACTATCCCACACCTGACAGTAATAGTCGGCC10180.13674837090779157No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCC10010.13446475371188543No Hit
GCCCTCAGGGATCCCTGAGCGAGTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGA10000.13433042328859685No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10000.13433042328859685No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG9850.13231546693926788No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC9810.13177814524611348No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATAAGGGATCACTATCACTATCCCAC9670.12989751932007312No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGACTCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCAT9420.1265392587378582No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGC9410.12640492831456962No Hit
CACTATCACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG9300.12492729365839504No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG9170.12318099815564329No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9140.12277800688577749No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG9010.12103171138302574No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG8940.12009139842000555No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA8870.11915108545698538No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCA8860.11901675503369678No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGAGTCTCTGGCTCCAACTCTGGGAAC8720.11713612910765642No Hit
GTGTGGGATAGTGATAGTGATCCCTTATATGTCTTCGGAACTGGGACCAA8600.11552416402819327No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8240.11068826878980378No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGAGTCTCTGGCTCC8230.1105539383665152No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC8070.10840465159389764No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG8000.10746433863087745No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT7970.10706134736101167No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAG7930.10652402566785728No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA7860.1055837127048371No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7850.10544938228154851No Hit
CTATTACTGTCAGGTGTGGGATAGTGATAGTGATCCCTTATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCT7640.10262844339248797No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGAGTCTCTGGCTCCAACTCTGGGAACACGGCCACC7500.10074781746644762No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA7470.10034482619658183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1850.067.32857570
GTACTAA200.00793112650.777421
TCTCGCG2507.2759576E-1249.82314370
CGTATGC1150.047.7005641
TCGTATG1150.047.6486740
GTGGTAT1850.045.7454221
TTCTGAT4100.045.56995470
TCTCGTA1100.043.50488738
GGTATCA6850.043.48821
ATGCCGT1350.040.7781444
ACGAGAC1350.040.10425622
TGGTATC2150.039.3491172
TCCCAGT18900.038.8831470
CACGAGA1450.037.33844821
GTATCAA18200.036.083591
AAAACCG1400.002225231635.58796370
AGGTAAT10750.034.76033470
GACTAAC400.002856304433.8334247
TATCAAC19800.032.472962
GTATGCC1800.032.41758342
Produced by FastQC (version 0.11.5)