FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763373
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGA56730.7431491551312399No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTCCCAGAGCCACTGCC35550.46569632407748246No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT34490.45181058276884306No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33400.43753184878165724No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC31190.4085813881287392No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA24070.3153111257537272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23300.3052243136710363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23300.3052243136710363No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23160.30339034783781976No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTCCCAGAGCCACTGCC22160.29029059188627315No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAGCGGCTACTTAGCCTGGTACC20920.27404689450635533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG20830.2728679164707161No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19510.2555762386146746No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18880.24732339236520026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA18880.24732339236520026No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGC18450.2416904973060352No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18120.2373675778420248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18120.2373675778420248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17600.23055570474722056No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17260.22610178772369469No Hit
GAATTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTA17240.2258397926046638No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17050.22335083897386993No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16790.2199449024264678No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16760.21955190974792138No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16160.21169205617699344No Hit
CACTAGGGCCGGTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCACC15840.2075001342724985No Hit
GTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15790.20684514647492117No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15460.2025222270109108No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15420.20199823677284892No Hit
GCCCTAGTGGATGCATGAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCCG15100.19780631486835398No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC14320.18758850522614762No Hit
GAGTGAATTCTGTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCACCGGCCCTAGTGGATGCATGAA14200.18601653451196204No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGATGAGCCACCATACTGCTGAC13690.17933565897667328No Hit
GTCTGGGATGCCACCGGCCCTAGTGGATGCATGAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGT13640.17868067117909592No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13610.17828767850054952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13280.17396475903653916No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTC12440.16296096403723997No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC12220.16007901772789973No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11700.1532671446330955No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11560.15143317879987894No Hit
CACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGGCTCATCTTCCACTTT11550.1513021812403635No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTCATGCATCCACTAGGGCCGGTGGCATCCCAGACAGGTT11450.14999220564520882No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCAC11290.14789624469296136No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11140.14593128130022937No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10930.1431803325504046No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10900.14278733987185818No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10900.14278733987185818No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10880.14252534475282724No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC10850.14213235207428088No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10560.13833342284833233No Hit
GGTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGT10470.13715444481269312No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10260.13440349606286833No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGATGAGCC10150.1329625229081982No Hit
CCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTCCC9920.12994957903934248No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC9880.12942558880128063No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA9820.12863960344418784No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9710.12719863028951772No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG9630.126150649813394No Hit
CCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC9610.12588865469436306No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9570.12536466445630118No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC9520.12470967665872384No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTCCAGGCACC9450.12379269374211557No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT9390.12300670838502278No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9200.12051775475422893No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC9170.12012476207568254No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTT9150.1198627669566516No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9150.1198627669566516No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCCGCTGCTAACAGTCTGACTGGCCCTGCAGG9140.11973176939713613No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC9120.11946977427810519No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9020.11815979868295054No Hit
GTCAGACTGTTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTC8860.11606383773070307No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGCCGCTGCTAACAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC8810.11540884993312575No Hit
CTGTTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTCATGCAT8800.11527785237361028No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8720.11422987189748654No Hit
TCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTAT8720.11422987189748654No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC8700.1139678767784556No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTCATGCATCCACTAGGGCCGGTGGCATC8680.11370588165942469No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8610.11278889874281643No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8520.11160992070717722No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8390.10990695243347616No Hit
CCACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTC8350.10938296219541431No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8240.10794198904074416No Hit
ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACG8210.10754899636219778No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCC8200.10741799880268231No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8140.10663201344558951No Hit
CTCCCAGGCTCCTCATCTTTCATGCATCCACTAGGGCCGGTGGCATCCCA8130.10650101588607405No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT8080.10584602808849672No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8070.10571503052898125No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC8070.10571503052898125No Hit
GCAGTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8040.10532203785043484No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7940.10401206225528019No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7860.10296408177915646No Hit
GTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCACCGGCCCTAGT7810.10230909398157913No Hit
GAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC7800.10217809642206366No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT7730.1012611135054554No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG7680.10060612570787807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)