FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763373
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGA50960.6675635632908159No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34010.4455226999121006No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTCCCAGAGCCACTGCC32980.4320299512820076No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC29160.38198888354709953No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT28650.37530800801181075No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24040.3149181330751808No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA23230.304307330754428No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21260.2785008115298812No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTCCCAGAGCCACTGCC21230.2781078188513348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20720.271426943316046No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19970.26160212635238606No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAGCGGCTACTTAGCCTGGTACC19040.2494193533174477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19010.24902636063890132No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18290.23959453635378775No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18110.23723658028250932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17450.22859074135448856No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16960.22217186093823074No Hit
GAATTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTA16830.22046889266452968No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16390.21470500004584914No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16280.21326402689117901No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGC16170.21182305373650892No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15610.2044871904036428No Hit
GCCCTAGTGGATGCATGAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCCG15580.20409419772509638No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15100.19780631486835398No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14940.19571035391610656No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14690.1924354149282199No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14540.1904704515354879No Hit
GAGTGAATTCTGTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCACCGGCCCTAGTGGATGCATGAA14510.1900774588569415No Hit
CACTAGGGCCGGTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCACC14480.1896844661783951No Hit
GTATGGTGGCTCATCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14350.18798149790469404No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13920.18234860284552898No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC13900.18208660772649807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC13760.18025264189328152No Hit
GTCTGGGATGCCACCGGCCCTAGTGGATGCATGAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGT13720.17972865165521965No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGATGAGCCACCATACTGCTGAC13600.17815668094103407No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG13560.17763269070297222No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13320.174488749274601No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13130.17199979564380716No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC11790.1544461226687347No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCAC11710.15339814219261094No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11400.14933721784763151No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTC11350.14868223005005415No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11260.14750325201441497No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11010.1442283130265283No Hit
CACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGGCTCATCTTCCACTTT10840.1420013545147654No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10750.14082237647912618No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC10660.139643398443487No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGCCGCTGCTAACAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC10640.13938140332445606No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10460.13702344725317767No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10410.13636845945560033No Hit
GGTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGT10120.13256953022965182No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10090.13217653755110542No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG9860.1291635936822497No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTCATGCATCCACTAGGGCCGGTGGCATCCCAGACAGGTT9860.1291635936822497No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCCGCTGCTAACAGTCTGACTGGCCCTGCAGG9560.12523366689678572No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA9460.12392369130163104No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9130.11960077183762066No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9060.1186837889210124No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC9050.11855279136149693No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC8990.11776680600440413No Hit
CCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAATTCTGTCCC8990.11776680600440413No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG8980.11763580844488866No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8850.1159328401711876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8840.11580184261167213No Hit
CTGTTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTCATGCAT8690.11383687921894016No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC8590.11252690362378549No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8570.11226490850475455No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8520.11160992070717722No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC8390.10990695243347616No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC8390.10990695243347616No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8370.10964495731444522No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTCCAGGCACC8280.10846597927880604No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8280.10846597927880604No Hit
CTCCCAGGCTCCTCATCTTTCATGCATCCACTAGGGCCGGTGGCATCCCA8270.10833498171929057No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTCATGCATCCACTAGGGCCGGTGGCATC8230.10781099148122869No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8220.10767999392171325No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8180.10715600368365137No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC8130.10650101588607405No Hit
CCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC8060.10558403296946578No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGATGAGCC8060.10558403296946578No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTT8010.10492904517188846No Hit
GGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTC8010.10492904517188846No Hit
GTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCACCGGCCCTAGT7930.10388106469576472No Hit
GTCAGACTGTTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTC7870.10309507933867193No Hit
TCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTAT7820.10244009154109458No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7780.10191610130303272No Hit
GTTCAGTGGCAGTGGCTCTGGGACAGAATTCACTCTCACCATCACCAGAC7650.10021313302933166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)