FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893574
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCGTATTCTTGGAATTGTCTCTGG48470.5424284950099265No Hit
GTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGGTTATTATGCCTACTTTGACGACTGGGGC26540.29700953698294713No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTC23050.2579528947798392No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGACAATTCCAAGAATACGGTGTATCT20920.2341160329194896No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20670.23131827918001194No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA17480.19561894146427716No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17320.19382837907101147No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGCTTCTTTTATATGAGCTGCAGTAATAATC16890.18901624263910993No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16570.18543511785257852No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16520.184875567104683No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16470.18431601635678746No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16290.18230163366436355No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAGCAGAATTCAGGCAAAGC16100.18017534082236053No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15900.1779371378307784No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15670.175363204390459No Hit
GTATGTGGTAGCACCACTACCACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT15270.17088679840729476No Hit
CTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15230.17043915780897834No Hit
GTAGATACACCGTATTCTTGGAATTGTCTCTGGAGAGGGTGAACCGGCCC14790.16551511122749768No Hit
CTCCAGAGACAATTCCAAGAATACGGTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACT14330.1603672443468588No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGT14060.15734567030822294No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13720.15354072522253334No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13440.15040724103431838No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG13290.14872858879063178No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13050.14604274520073324No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12780.14302117116209737No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC12750.14268544071336006No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12640.1414544290679899No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC12570.14067105802093616No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12560.14055914787135704No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12530.14022341742261973No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12530.14022341742261973No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG12440.1392162260764078No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA12410.13888049562767046No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT12400.13876858547809134No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12350.1382090347301958No Hit
GATTATTACTGCAGCTCATATAAAAGAAGCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC12070.13507555054198087No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12020.13451599979408532No Hit
GTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCG11850.1326135272512405No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11440.12802521111849718No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11390.12746566037060164No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC11290.12634655887481058No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC11190.12522745737901952No Hit
GCCGTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGG11110.12433217618238669No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11010.12321307468659563No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10810.1209748716950135No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10800.12086296154543441No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10750.12030341079753888No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCAAAGGTGCTAATGGTGAATCCAGAGGCTGCAC10710.11985577019922244No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10700.11974386004964335No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10600.1186247585538523No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCAGGCCCCAGTCGTCAAAGTAGGCATAAT10560.11817711795553586No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10340.11571509466479554No Hit
CCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10280.1150436337673209No Hit
GTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACTACTGTGCGAAAAGTCCCTTGCATT10100.11302925107489697No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10090.11291734092531788No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10030.11224588002784325No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG10000.11191014957910592No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC9960.11146250898078951No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9920.11101486838247308No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTCAGTGTGAGGTGCAGCTGTTGGAGT9900.11079104808331486No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT9840.11011958718584022No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA9740.10900048569004916No Hit
GTACTATCATAGTAATGCAAGGGACTTTTCGCACAGTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCAT9640.1078813841942581No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9640.1078813841942581No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG9550.10687419284804615No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC9550.10687419284804615No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG9510.10642655224972974No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCC9410.10530745075393869No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG9350.10463598985646404No Hit
GTTTGGGGGCTTTGCCTGAATTCTGCTGGTACCAGGAGACATACTTATAA9250.10351688836067298No Hit
GTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATA9190.10284542746319834No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT9140.10228587671530281No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACC9100.10183823611698639No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9090.10172632596740729No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9030.10105486506993265No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9000.10071913462119533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)