FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893574
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCGTATTCTTGGAATTGTCTCTGG45150.5052743253496633No Hit
GTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGGTTATTATGCCTACTTTGACGACTGGGGC24170.27048683153269903No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTC21270.2380328881547583No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGACAATTCCAAGAATACGGTGTATCT20830.23310884157327766No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17630.19729759370796374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16510.18476365695510388No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15780.17659421603582914No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGCTTCTTTTATATGAGCTGCAGTAATAATC15730.17603466528793363No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15690.1755870246896172No Hit
CTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15390.17222972020224403No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15320.17144634915519028No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAGCAGAATTCAGGCAAAGC15120.16920814616360816No Hit
GTATGTGGTAGCACCACTACCACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT14940.16719376347118425No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14790.16551511122749768No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14760.16517938077876035No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14560.16294117778717823No Hit
CTCCAGAGACAATTCCAAGAATACGGTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACT14400.16115061539391254No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGT14330.1603672443468588No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14140.1582409515048558No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14130.1581290413552767No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14070.15745758045780206No Hit
GTAGATACACCGTATTCTTGGAATTGTCTCTGGAGAGGGTGAACCGGCCC13630.15253353387632138No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13410.15007151058558105No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG13230.14805712789315714No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12550.14044723772177792No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC12510.13999959712346152No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG12330.13798521443103762No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12210.13664229263608835No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA12190.1364184723369301No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11770.1317182460546077No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCAAAGGTGCTAATGGTGAATCCAGAGGCTGCAC11630.13015150396050018No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT11450.12813712126807628No Hit
GTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCG11410.12768948066975988No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11250.12589891827649416No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11170.1250036370798613No Hit
GATTATTACTGCAGCTCATATAAAAGAAGCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC11110.12433217618238669No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10890.12187015289164635No Hit
GCCGTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGG10750.12030341079753888No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10610.11873666870343139No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10580.11840093825469407No Hit
GTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACTACTGTGCGAAAAGTCCCTTGCATT10520.11772947735721943No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA10490.11739374690848212No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC10480.11728183675890301No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10400.11638655556227018No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10090.11291734092531788No Hit
CCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10070.11269352062615967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10010.11202205972868504No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10000.11191014957910592No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCAGGCCCCAGTCGTCAAAGTAGGCATAAT9980.11168632927994772No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9840.11011958718584022No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9790.1095600364379447No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9650.10799329434383721No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9640.1078813841942581No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9540.10676228269846705No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9430.10553127105309688No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT9420.10541936090351779No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC9190.10284542746319834No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG9180.10273351731361924No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9140.10228587671530281No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCC9100.10183823611698639No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC9080.10161441581782818No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTCAGTGTGAGGTGCAGCTGTTGGAGT9070.10150250566824909No Hit
GTACTATCATAGTAATGCAAGGGACTTTTCGCACAGTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCAT8990.10060722447161623No Hit
GTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATA8970.100383404172458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG150.002663377466.8940054
TGGTATC1450.048.4676132
CTGCAGT11650.044.32110270
GTGGTAT1750.042.1183741
TCTCGCG6100.038.09071470
GTATCAA13650.036.8167571
GACAGGT1104.0017767E-1133.503251
ATTATAC400.003022879433.445133
GAGCCAT13100.032.51764770
TATCAAC15500.031.9543742
GGTCAGT8250.031.29338370
AAGCGGT8350.030.91861270
TCAACGC16350.030.4807554
ATCAACG16600.030.0200273
GCTTGCA450.005334631529.7806661
CAACGCA16750.029.757855
CTTAAAT1750.00557411729.50519270
CCACGTG10500.029.50519270
ATGTGAT2204.8049906E-429.33754770
AACGCAG17000.029.3235196
Produced by FastQC (version 0.11.5)