FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640158
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGG67410.4109969893144441No Hit
ATGTACTACTGTGCGAGGTGGTATTATTATGATAGTAATGGACGGGGTGCTTTTGATATCTGGGGCCAAGGGACA47770.29125242811972996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45590.27796102570606No Hit
GTATTACTGTCAGCAGTGTACTCACTCACCCTGGACGTTCGGCCAAGGGA45230.27576611521572925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT42240.25753616419881503No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG37610.2293071765037271No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC34920.21290631756208855No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC33160.20217564405380456No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATC33030.20138303748785177No Hit
CAGTAGTACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA31210.1902865455645127No Hit
CCTTTAGTACTGATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGC31130.1897987876777725No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30350.18504314828205576No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30240.18437248118778798No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG28430.17333695900029145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG27530.1678496827744644No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27260.1662034999067163No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC27170.1656547722841336No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG26880.16388664994470045No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26750.16309404337874767No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26680.16266725522785No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26530.16175270919021217No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATCAGTACTAAAGGTGAATCCAGAGGCTGCAC26320.1604723447375192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG25750.15699706979449543No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTATC25690.15663125137944026No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25670.15650931190775524No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA25350.15455828036079453No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25240.15388761326652678No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24910.1518756119837235No Hit
GTCTGGGACAGACTTCACTCTCACTATCAACAGACTGGAGCCTGAAGATT24560.14974167122923523No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTACTGATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG24180.14742482126721937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24020.14644930549373902No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTG23340.1423033634564475No Hit
GTACTACTGTGCGAGGTGGTATTATTATGATAGTAATGGACGGGGTGCTT23110.14090105953206947No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTATGTACT22190.13529184383455742No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22160.13510893462702983No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC22140.1349869951553448No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22060.1344992372686046No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22050.13443826753276208No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGC22020.1342553583252345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG22020.1342553583252345No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG21980.13401147938186445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21940.13376760043849434No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21570.13151172021232102No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG21130.12882905183525No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT20870.12724383870334444No Hit
GTACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG20660.12596347425065146No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20520.12510989794885613No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC20040.12218335062841508No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG19940.12157365326998984No Hit
GTGTACTCACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19820.12084201643987957No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC19760.12047619802482443No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA19470.1187080756853913No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTC19460.11864710594954876No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCC19410.11834225727033615No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC19380.11815934806280859No Hit
GCTATGTACTACTGTGCGAGGTGGTATTATTATGATAGTAATGGACGGGGTGCTTTTGATATCTGGGGCCAAGGG19290.1176106204402259No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18360.11194043500687129No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC18110.11041619161080822No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGTGAGTGAGTACACTGCTGAC18050.11005037319575309No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT18040.10998940345991057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17970.1095626153090129No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17800.10852612979969004No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT17720.10803837191294985No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG17700.10791643244126481No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC17450.10639218904520174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17270.10529473380003634No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17090.10419727855487093No Hit
GTGTATTACTGTCAGCAGTGTACTCACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA17070.10407533908318588No Hit
GTCTCTGGAGATGGTGAATCGGCCCTCCAGAAATTCATTATAGTATGTGCCACTTTCATCTTGCTTTATGTTGGC16920.10316079304554807No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT16660.10157557991364247No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGAAAGTGGCACATACTATAATGAATTTCTGGAGGGCCGATTC16610.10127073123442985No Hit
GTAATGGACGGGGTGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCC16540.10084394308353219No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCG16510.10066103387600463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCAT21650.040.95828670
AACTGTG31600.040.70909570
TACTCAT1300.001528119138.42889870
TCTCGCG22050.037.94962770
GTATCAA35000.036.547241
ATGTGAT3305.862603E-934.06197770
TCGCTAA1004.474714E-1033.98721331
CCTATAC601.5356447E-533.8307533
CTTATAC1156.002665E-1132.368731
ATCAACG41000.031.1903043
CGTATGC1101.1277734E-931.17917841
TCAACGC41200.030.9567814
CGCTAAT1101.2350938E-930.91924732
GACAATG17850.030.78617570
CCACGTG15900.030.6343670
CAACGCA41900.030.3579355
GTGGCCG23700.030.03777970
ACTCAGT19700.029.79702870
TCGTATG1151.8335413E-929.79593540
AACGCAG43100.029.5939036
Produced by FastQC (version 0.11.5)