FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640158
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGG60980.3717934491677022No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45950.28015593619639084No Hit
ATGTACTACTGTGCGAGGTGGTATTATTATGATAGTAATGGACGGGGTGCTTTTGATATCTGGGGCCAAGGGACA43190.26332828910385464No Hit
GTATTACTGTCAGCAGTGTACTCACTCACCCTGGACGTTCGGCCAAGGGA40510.24698839989805862No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT38200.2329043909184359No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATC32110.19577382179033972No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31810.19394472971506405No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC30970.18882327190429216No Hit
CCTTTAGTACTGATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTGGCCAACATAAAGC29940.18254338911251233No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29700.1810801154522918No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG28820.1757147786981498No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC28810.17565380896230728No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27740.16913004722715738No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27550.16797162224614945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG27360.16681319726514154No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26990.1645573170389682No Hit
CAGTAGTACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA26570.16199658813358225No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG25940.15815549477550334No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24930.15199755145540855No Hit
GTCTGGGACAGACTTCACTCTCACTATCAACAGACTGGAGCCTGAAGATT24800.15120494488945577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24750.15090009621024314No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTACTGATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG24730.15077815673855813No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATCAGTACTAAAGGTGAATCCAGAGGCTGCAC24600.14998555017260534No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC23930.14590057787115632No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG23900.14571766866362876No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23220.1415717266263372No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTATC23190.14138881741880965No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG22920.13974263455106153No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22040.13437729779691956No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21540.13132881100479343No Hit
GTACTACTGTGCGAGGTGGTATTATTATGATAGTAATGGACGGGGTGCTT21260.12962165840120282No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG20790.12675608081660425No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA20720.1263292926657066No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTATGTACT20600.12559765583559632No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20470.12480504926964354No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTG20330.12395147296784823No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGC20040.12218335062841508No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19750.12041522828898192No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT19730.12029328881729687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19720.12023231908145436No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC19620.11962262172302912No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19560.11925680330797399No Hit
GTGTACTCACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19390.11822031779865111No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19150.11675704413843055No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19040.11608637704416283No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18850.1149279520631549No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGTGAGTGAGTACACTGCTGAC18120.11047716134665074No Hit
GTGTATTACTGTCAGCAGTGTACTCACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA18040.10998940345991057No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17960.1095016455731704No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17920.1092577666298003No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17910.10919679689395778No Hit
GCTATGTACTACTGTGCGAGGTGGTATTATTATGATAGTAATGGACGGGGTGCTTTTGATATCTGGGGCCAAGGG17910.10919679689395778No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTC17900.10913582715811525No Hit
GTACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG17870.10895291795058769No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17760.10828225085631994No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17630.10748964429036716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17620.10742867455452462No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17170.10468503644161112No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT16750.10212430753622516No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGAAAGTGGCACATACTATAATGAATTTCTGGAGGGCCGATTC16750.10212430753622516No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16750.10212430753622516No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCC16470.10041715493263453No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC16440.10023424572510697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)