FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004693022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725409
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAAACTGAGGTGTCAGACTTGGAGACAG36100.4976502910771717No Hit
GATTATTACTGTGCAACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCT34500.47559376848095347No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28580.39398463487494645No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAAT28180.38847050422589186No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26440.36448403590250467No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGG25580.35262865500703744No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25190.34725237762420924No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24330.335396996728742No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA20790.2865969404846094No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC19490.26867601587518214No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18990.261783352563864No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18570.2559935153823567No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT18550.25571780884990397No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17610.24275960182462583No Hit
CCCCTGAGGGCCGCTGATTATCACTAGCGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGTTGGTACCAATTT17200.23710761790934495No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16820.23186919379274312No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCAACTCC16600.22883642193576312No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC16150.2226330249555768No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16130.22235731842312403No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT15880.21891098676746498No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15710.2165674812416168No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC15400.21229402998859953No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14900.20540136667728134No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAAACTGAGGTGTCAGACTTGGAGACAGAGAATCGGTCAGGGACC14470.19947367622954776No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG14290.19699231743747322No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14200.19575163804143592No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14130.1947866651778514No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14100.1943731053791723No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14090.19423525211294593No Hit
GATTATCACTAGCGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGTTGG14030.19340813251558775No Hit
ACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG13790.19009965412615504No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAATA13660.18830756166521231No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA13530.1865154692042696No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12750.17576291443861325No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12560.17314370238031235No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12540.17286799584785964No Hit
CTCTTGTTCTGGAGGCAACTCCAACATCGGAGGTAATGTTGTAAATTGGTACCAACAGTTTCCAGGAACGGCCCC12510.17245443604918054No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC12330.16997307725710598No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG12320.16983522399087964No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12250.1688702511272951No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGAC12130.16721601193257873No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTC12020.16569962600408872No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA11570.15949622902390237No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGTCTGACACCTCAGTTTCCCTGGCCATCAGTGGGCTCCAGT11260.1552227777708851No Hit
CCTTGGTCCCTCCGCCGAATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAATAATCAGCCTCATCCT11180.1541199516410742No Hit
ATGTTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG11160.15384424510862146No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11150.15370639184239512No Hit
ATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAATAATCAGCC10900.15026006018673604No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10870.14984650038805694No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10800.1488815275244724No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC10740.14805440792711422No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10700.14750299486220877No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC10530.14515948933636058No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10370.14295383707673878No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10350.14267813054428605No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGAC10150.1399210652197588No Hit
GTCCCATGTTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAAACTGAGGTGTC10070.13881823908994786No Hit
CTCCAACATCGGAGGTAATGTTGTAAATTGGTACCAACAGTTTCCAGGAA9700.13371766823957243No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA9500.13096060291504516No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9370.12916851045410244No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9360.1290306571878761No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGG9290.12806568432429155No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAG9280.12792783105806518No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG9200.12682500492825427No Hit
GTGCAACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCA9100.12544647226599065No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9060.12489505920108518No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9010.12420579286995337No Hit
GTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC8980.12379223307127428No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTC8790.12117302101297338No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8760.12075946121429429No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT8730.1203459014156152No Hit
CTGATGGCCAGGGAAACTGAGGTGTCAGACTTGGAGACAGAGAATCGGTC8700.11993234161693611No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8590.1184159556884461No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8510.11731312955863521No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8500.11717527629240884No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8450.11648600996127703No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC8450.11648600996127703No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC8430.11621030342882428No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATCACTAGCGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGTTGGT8390.11565889036391883No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG8280.11414250443542884No Hit
GTTTGGGGGCCGTTCCTGGAAACTGTTGGTACCAATTTACAACATTACCTCCGATGTTGGAGTTGCCTCCAGAAC8250.11372894463674975No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGTCTGACACCTCAGTTTCCCTGGCCATCAGTGGGCTCC8190.11290182503939157No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCA8170.11262611850693884No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8120.11193685217580703No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8080.11138543911090157No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8020.11055831951354339No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGACCACTC7870.10849052052014795No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7840.10807696072146886No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC7810.10766340092278977No Hit
ATTTACAACATTACCTCCGATGTTGGAGTTGCCTCCAGAACAAGAGATGGTGACCGTCTGCCCGGGGGTCCC7790.10738769439033705No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG7780.10724984112411068No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC7670.10573345519562068No Hit
GTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCAACT7620.10504418886448887No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGG7500.10338994966977251No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGG7440.10256283007241433No Hit
GACTGGAGCCCACTGATGGCCAGGGAAACTGAGGTGTCAGACTTGGAGAC7410.10214927027373524No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAA7270.10021932454656614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)