FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725409
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCT32710.4509180338264344No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC29750.41011346702343093No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25040.34518457863081375No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22970.3166489525219566No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22710.3130647676000711No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT22330.3078263434834693No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAAT21060.29031897867272116No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA20830.28714835354951485No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCAACTCC20220.27873930430970667No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGG19390.26729748321291846No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT17540.24179462896104126No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16910.2331098731887804No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16790.23145563399406405No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16390.2259415033450095No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16090.22180590535821862No Hit
CCCCTGAGGGCCGCTGATTATCACTAGCGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGTTGGTACCAATTT15820.2180838671701068No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC15740.2169810410402959No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15500.21367256265086318No Hit
CTCTTGTTCTGGAGGCAACTCCAACATCGGAGGTAATGTTGTAAATTGGTACCAACAGTTTCCAGGAACGGCCCC15100.20815843200180864No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14730.2030578611514332No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14600.20126576869049045No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC14600.20126576869049045No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAAACTGAGGTGTCAGACTTGGAGACAG14520.20016294256067954No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC14290.19699231743747322No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC14150.1950623717103041No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTC13900.19161604005464503No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13700.18885897473011778No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13600.18748044206785414No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGAC13290.18320699081483688No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGTCTGACACCTCAGTTTCCCTGGCCATCAGTGGGCTCCAGT12950.17851997976314052No Hit
GATTATCACTAGCGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGTTGG12930.17824427323068778No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12900.1778307134320087No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12720.17534935463993417No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12380.1706623435882378No Hit
CTCCAACATCGGAGGTAATGTTGTAAATTGGTACCAACAGTTTCCAGGAA12290.16942166419220056No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12210.16831883806238962No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12060.1662510390689942No Hit
ACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG11890.163907533543146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11700.1612883214848451No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG11390.15701487023182784No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11280.15549848430333785No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA11010.15177644611522603No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC10880.1499843536542833No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10880.1499843536542833No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10800.1488815275244724No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAG10800.1488815275244724No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10710.14764084812843514No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10540.14529734260258695No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA10530.14515948933636058No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC10460.14419451647277606No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10070.13881823908994786No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9960.13730185316145788No Hit
GTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCAACT9960.13730185316145788No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9910.13661258683032607No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTC9740.1342690813044779No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9700.13371766823957243No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAATA9630.13275269537598788No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9520.1312363094474979No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA9480.13068489638259245No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC9450.13027133658391335No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGG9440.13013348331768698No Hit
GTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9290.12806568432429155No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9240.1273764179931597No Hit
GTTGTAAATTGGTACCAACAGTTTCCAGGAACGGCCCCCAAACTCCTCATCGCTAGTGATAATCAGCGGCCCTC9180.12654929839580153No Hit
GTTTGGGGGCCGTTCCTGGAAACTGTTGGTACCAATTTACAACATTACCTCCGATGTTGGAGTTGCCTCCAGAAC9170.1264114451295752No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT9030.12448149940240609No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGG9000.124067939603727No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCA8880.12241370040901065No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8720.12020804814938883No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGAC8700.11993234161693611No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC8650.11924307528580427No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8530.11758883609108792No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG8430.11621030342882428No Hit
GTGCAACATGGGACGACAGCCTGAGTGGTCCGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCA8260.11386679790297613No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG8200.11303967830561792No Hit
GTCCCTGACCGATTCTCTGTCTCCAAGTCTGACACCTCAGTTTCCCTGGC8100.1116611456433543No Hit
CTGATGGCCAGGGAAACTGAGGTGTCAGACTTGGAGACAGAGAATCGGTC8010.11042046624731704No Hit
GAGGTAATGTTGTAAATTGGTACCAACAGTTTCCAGGAACGGCCCCCAAA8010.11042046624731704No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGTCTGACACCTCAGTTTCCCTGGCCATCAGTGGGCTCC7920.10917978685127976No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7840.10807696072146886No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC7790.10738769439033705No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGTTCTGGAGGCA7780.10724984112411068No Hit
GTCTGACACCTCAGTTTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG7770.10711198785788431No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG7750.10683628132543159No Hit
CTCCAAGTCTGACACCTCAGTTTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGC7730.10656057479297885No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGG7640.10531989539694159No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATCACTAGCGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGTTGGT7630.10518204213071523No Hit
ATATCGGACCACTCAGGCTGTCGTCCCATGTTGCACAGTAATAATCAGCC7610.1049063355982625No Hit
GCCCCCAAACTCCTCATCGCTAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGT7590.10463062906580978No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7530.1038035094684516No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC7530.1038035094684516No Hit
GTCACCATCTCTTGTTCTGGAGGCAACTCCAACATCGGAGGTAATGTTGTAAATTGGTACCAACAGTTTCCAGGA7480.10311424313731976No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC7380.10173571047505615No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7360.1014600039426034No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7350.10132215067637704No Hit
ATTTACAACATTACCTCCGATGTTGGAGTTGCCTCCAGAACAAGAGATGGTGACCGTCTGCCCGGGGGTCCC7320.10090859087769795No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAA7320.10090859087769795No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCGGACCACTC7260.10008147128033977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAATT750.00836261750.0874270
TAAGAAT1353.246175E-546.37724370
GTATTGC452.1966862E-644.9032631
GTGGTAT2500.044.454231
GGTATCA6450.041.248351
GTATCAA16650.039.2397881
TGGTATC2750.039.1585662
CCTAGAG558.808043E-636.6858523
CATGTTG14750.036.50439570
ATAAGCC400.002195363435.69819362
TATCAAC18750.034.639022
CTTATGG650.002619639734.43242369
CTCCGAT11700.034.2478170
CTAGACA502.163332E-433.6263774
ATCAACG19650.033.2008483
TCAACGC20000.032.2813224
CAACGCA20050.032.203045
AACGCAG20600.031.1800066
CCAATAC450.00523741229.8921783
TGGTCTA450.00523741229.8921785
Produced by FastQC (version 0.11.5)