FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414038
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43970.3109534538675764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT38540.27255278853892184No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGACTGCTCAGTTCCATGTAGGCCGTGGTCGCGGATGTGTTCCTGGT34990.24744738118777573No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32950.23302061189303258No Hit
GTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCAACTGGTTCGACCC30780.2176744896530362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29550.208975996401794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28110.1987923945466812No Hit
TCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACT27470.19426634927774217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG26620.18825519540493252No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24370.1723433175063188No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG24150.170787489445121No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23470.16597856634687327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23030.1628669102244777No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCACCTATTTAAGTTGGTATCAGCAGCA22890.1618768378218973No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTA22890.1618768378218973No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCAC22700.160533168132681No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22360.15812870658355715No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGAC21390.15126891922282146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC21140.14950093278964213No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT21040.14879373821637043No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG19880.14059028116641842No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC19550.13825653907462176No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT19470.13769078341600438No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTAT19290.1364178331841153No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTG19020.13450840783628162No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18980.13422553000697293No Hit
ACATGGAACTGAGCAGTCTGAGATCTGAAGACACGGCTGTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCA18890.1335890548910284No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18870.13344761597637406No Hit
CACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAA18350.1297702041953611No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18230.12892157070743504No Hit
GTGGATGCACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTAATG18150.12835581504881763No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGA17760.1255977562130579No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17600.12446624489582316No Hit
ACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA17540.12404192815186013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17480.1236176114078971No Hit
GAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTAT17460.12347617249324275No Hit
ATATAATACATTGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGATGGATGAACGCTGGCAATGGTC17170.12142530823075477No Hit
CATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTC17150.12128386931610043No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16990.12015235799886566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16430.11619206838854401No Hit
GTTCCATGTAGGCCGTGGTCGCGGATGTGTTCCTGGTAAAGGTGACTGTG15490.10954443939978982No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA15470.10940300048513547No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15460.1093322810278083No Hit
CTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACT15450.10926156157048113No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC15450.10926156157048113No Hit
CCCCTAACCTCCTCATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCA15360.10862508645453658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15340.10848364753988224No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15230.10770573350928335No Hit
GTCCCAGACACACTGCCTGTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACTATGGATGAGGAGGTT15210.10756429459462899No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGCATACAGCTTCAGTAC15190.10742285567997464No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15170.1072814167653203No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14910.10544271087481383No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14850.1050183941308508No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC14470.10233105475241826No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC14460.10226033529509108No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14320.10127026289251068No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC14280.10098738506320198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)