FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287249
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT137141.0653727445117456No Hit
ATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA94300.7325700000543796No Hit
GTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC89840.6979224687686687No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT81530.6333661941085214No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79040.6140226172247949No Hit
GTTTTATACAGCTCCAACACTAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTC75300.5849684093753423No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT67070.5210336150970014No Hit
GCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTAGTGTTGGAGCTGTATAAAACATT65220.5066618812677268No Hit
GAGTAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT63450.4929116278202586No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48920.380035253474658No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46560.36170158221136706No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCTTCTCGCCCAGAGACACAGCCAGGGAGTCTGG46160.35859418030233464No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG44550.34608688761847944No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43550.3383183828458985No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTAT43380.33699773703455976No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG39860.30965260023507496No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC38540.2993981739352682No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA37110.2882892121104774No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC36030.27989922695609004No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35410.2750827539970899No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC33150.25752593321105705No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC32940.25589454720881505No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG32530.2527094602520569No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT32230.25037890882028263No Hit
GTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACACTAAGAACT31380.24377567976358885No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGT31090.24152281337954043No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30240.23491958432284663No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTAGTATAATACTGCTGAC28930.22474284307076564No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27880.21658591305955568No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCA27790.2158867476300234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA27530.21386693638915238No Hit
CCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAAC26640.20695296714155534No Hit
AAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA26510.20594306152111985No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT26510.20594306152111985No Hit
GCTCCAACACTAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG26420.20524389609158758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26410.20516621104386176No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTAGT26100.20275797456436165No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC25600.19887372217807123No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24650.19149364264411936No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTG24620.19126058750094194No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23380.18162764158294162No Hit
GTTTATTACTGTCAGCAGTATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA23070.17921940510344153No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTA23020.17883097986481247No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT22680.17618968824213496No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC22500.17479135738307042No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT22170.1722277508081187No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT22030.17114016013995736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC21880.16997488442407024No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21350.16585757689460237No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTACTCCGCTCACTTT21310.16554683670369913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21140.16422619089236037No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21080.16376008060600553No Hit
ACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATT20900.16236174974694095No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20690.16073036374469898No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20080.1559915758334246No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19970.1551370403084407No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA19940.1549039851652633No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19770.15358333935392454No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTACT19670.15280648887666645No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT19190.1490776065858276No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATAC19160.14884455144265019No Hit
GTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTAGTGTTGGAGCTGTATAAAACATTCTGGCTGGACTTGC18560.14418344857910162No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18490.14363965324502098No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18350.14255206257685965No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18320.14231900743368223No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATG18300.1421636373382306No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA18280.14200826724277898No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC18280.14200826724277898No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGG18080.1404545662882628No Hit
GAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGA17900.13905623542919823No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17880.1389008653337466No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT17840.13859012514284338No Hit
CTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC17670.13726947933150463No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17650.137114109236053No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTACTCCGC17160.13330754189748836No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGT16860.1309769904657141No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC16580.12880180912939143No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16520.12833569884303658No Hit
GGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACACTAAGAACTACTTAGCTTGGTACC16320.1267819978885204No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16020.12445144645674613No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15920.12367459597948803No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC15740.12227626512042347No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA15580.12103330435681053No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15440.1199457136886492No Hit
GTATATTACTGTGCGAGAGAGAGATTACATTTGGGGCAGTTAATGTTTTA15430.1198680286409234No Hit
GTGTTGGAGCTGTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGC15380.11947960340229434No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15270.11862506787731046No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15240.11839201273413302No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14890.11567303606372971No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14870.11551766596827807No Hit
CTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACACTAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGG14850.11536229587282647No Hit
ACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACACTAAGAACTACTTAGCTTGGTACCAGC14650.11380859491831029No Hit
GTAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14520.11279868929787477No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14470.11241026405924572No Hit
ATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA14330.1113226733910844No Hit
TACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGC14310.11116730329563279No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14290.11101193320018116No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT14270.11085656310472955No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC14140.10984665748429402No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14120.10969128738884242No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14100.10953591729339078No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14060.10922517710248755No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14060.10922517710248755No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGC13930.10821527148205204No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13910.1080599013866004No Hit
GTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTG13900.10798221633887461No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13860.10767147614797137No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC13860.10767147614797137No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13800.10720536586161653No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC13760.10689462567071328No Hit
GCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT13740.10673925557526166No Hit
GTAATATACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATATAGACTGTACTCGCGGATGTGTCCCTGGA13360.10378722376168092No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC13210.10262194804579379No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13200.10254426299806797No Hit
TTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTT13170.10231120785489055No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTC13110.10184509756853569No Hit
TTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGT13090.10168972747308408No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC13030.10122361718672922No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12980.10083519194810017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG351.7978455E-6150.0023270
CACCGTT200.009547388131.2520470
CGCAATT200.009547388131.2520470
CCCTATG653.8969094E-580.77048570
TGTACGT250.00254829767.642669
ACCCTGG5450.057.79905770
CGCCTTA252.17175E-457.1011664
GGTCTGG41300.053.07285770
CAGTTCG29300.047.03571370
GTGGTAT3000.046.1565861
TGGTATC2950.045.7833752
GGTATCA9850.045.25941
GGAAACG1453.7551596E-545.25932370
GCTTGTA1600.044.327211
CAGCCAT6550.042.08080770
GTATCAA25800.038.616541
CGTGTCG350.00149859138.5873410
CATTTGG7300.037.75743570
AATCTGT22700.037.5899568
AGAGTCG451.1372331E-437.51547211
Produced by FastQC (version 0.11.5)