FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287249
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT130661.0150328335854213No Hit
GTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC124380.9662466236136134No Hit
ATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA84740.6583030944285061No Hit
GAGTAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT81020.6294042566745051No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77470.6018260647318429No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT63980.4970289353497265No Hit
GCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTAGTGTTGGAGCTGTATAAAACATT59660.4634689947321769No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT58350.45329225348009594No Hit
GTTTTATACAGCTCCAACACTAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTC53570.4161588006671592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45120.35051493533885053No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45020.34973808486159247No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43340.3366869968436565No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA40430.31408064795544605No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG37530.29155198411496147No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCTTCTCGCCCAGAGACACAGCCAGGGAGTCTGG35520.2759372895220738No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTAT34550.26840183989267036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA34070.2646729576018315No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC33900.2633523117904928No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTAGTATAATACTGCTGAC32860.2552730668270086No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC32130.24960205834302454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC31640.2457954910044599No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG31310.2432318844295082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31250.24276577414315334No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTT29810.23157912727063684No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT29360.22808330012297545No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC28000.2175181336322654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG27650.21479915696186205No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC27330.2123132354346362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26970.20951657371650706No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT26480.2057100063779424No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGT26420.20524389609158758No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTAGT25920.2013596437052971No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25640.19918446236897444No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24540.19063910711913545No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC24490.19025068188050642No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC24410.18962920149869994No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24300.18877466597371603No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC24070.18698790987602243No Hit
GTTTATTACTGTCAGCAGTATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA23400.18178301167839322No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT22810.17719959386257048No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22770.17688885367166726No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC22280.1730822863331026No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22210.17253849099902194No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCA21810.16943108908998958No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA21680.16842118346955406No Hit
CCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAAC21440.16655674232413464No Hit
GTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACACTAAGAACT21410.16632368718095722No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTACTCCGCTCACTTT20660.16049730860152153No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20450.15886592259927956No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTA20430.15871055250382793No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19860.15428250478345681No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG19810.15389407954482778No Hit
GTAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT19610.15234037859031158No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTG19410.15078667763579542No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTACT19070.14814538601311789No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18650.14488261400863392No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18610.14457187381773068No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18240.14169752705187574No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC18230.14161984200414995No Hit
AAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA18190.14130910181324668No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18190.14130910181324668No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT18000.13983308590645632No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17920.13921160552464984No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17790.13820169990421433No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATG17670.13726947933150463No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT17600.13672568399742396No Hit
GTCCTGCTTTCTGCTGGTACCAAGCTAAGTAGTTCTTAGTGTTGGAGCTGTATAAAACATTCTGGCTGGACTTGC17530.1361818886633433No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17390.13509429799518197No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATACTACTCCGC17260.13408439237474645No Hit
GCTCCAACACTAAGAACTACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG17210.13369596713611742No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16610.12903486427256886No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTAGTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC16110.1251506118862784No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGG16100.1250729268385526No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16050.12468450159992354No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16000.12429607636129453No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC15900.12351922588403642No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGT15790.12266469035905252No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15670.12173246978634282No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15590.12111098940453635No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT15590.12111098940453635No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15450.12002339873637502No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15370.11940191835456854No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15330.1190911781636653No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC15190.11800358749550396No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAAGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATAC15100.11730442206597169No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15090.1172267370182459No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15070.11707136692279427No Hit
ACTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGCTGCTCATT14960.11621683139781036No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT14490.11256563415469735No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC14320.11124498834335858No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC14300.11108961824790697No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA14220.1104681378661005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13960.10844832662522945No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13930.10821527148205204No Hit
GTATATTACTGTGCGAGAGAGAGATTACATTTGGGGCAGTTAATGTTTTA13920.10813758643432622No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13900.10798221633887461No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT13700.10642851538435842No Hit
GTGTTGGAGCTGTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGC13670.10619546024118101No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13200.10254426299806797No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA12980.10083519194810017No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12950.10060213680492275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG33100.059.49045670
CAGTAGT6400.059.4135370
ACCCTGG6950.054.7117470
TCGTCAT750.006070965454.32094670
TAAGGTG1053.5321005E-451.73422670
GTGGTAT3000.049.4108351
TTCTAGT850.00995718747.9302470
TGGTATC3100.046.6993182
CAGTTCG32250.046.32018370
GGTATCA9550.043.7430531
GCTTGTA1650.038.793631
CATTTGG7750.036.79805870
GTATCAA23800.035.387821
CTTGTAT1450.034.827882
GTCCTTG40850.033.2441570
AGTTTAT29600.033.03370
TATCAAC25950.032.174972
AAGCGGT5650.031.2465670
CTCTCCG1750.004351935331.04053770
GTCGCTG2950.030.8341871
Produced by FastQC (version 0.11.5)