FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328932
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54660.4113077268061872No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT49680.3738340261202228No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTA40840.3073144449828885No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32640.24561076112246524No Hit
GTCTCCGGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCT32070.24132160261021632No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG30690.23093732410687678No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30030.22597093004006222No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29480.22183226831771677No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG29150.2193490712843095No Hit
GTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGCCTGGT28710.2160381419064331No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA28380.21355494487302587No Hit
ACTATATACTAATTGGCCCGCGCTCTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACC27890.2098677735203908No Hit
GTATATAGTTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTACTGC27690.2083628056213561No Hit
CTCCTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT27380.2060301053778523No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGCAGTAGAGTTC27010.20324591476463807No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25060.18857247774904962No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC24210.18217636417815208No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC24170.18187537059834513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG23730.17856444122046877No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23230.17480202147288199No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGAG22930.1725445696243299No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCC22090.1662237044483841No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC21630.16276227828060427No Hit
CTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT21600.16253653309574906No Hit
CACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCC21450.16140780717147304No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20580.15486119681067204No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA19540.14703536373569154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19210.14455216670228424No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18830.14169272769411828No Hit
CACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGCAGTAGAGTTCACTCTTACCATCAGC18810.1415422309042148No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18480.13905903387080754No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18260.13740356918186936No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC18190.13687683041720722No Hit
CTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAACTATATACTAATTGGCCCGCG17450.13130844919077878No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17320.1303302200564062No Hit
CTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT17300.1301797232665027No Hit
CCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGC17280.13002922647659926No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16710.12574006796435033No Hit
CTGCAGTAGAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAACTATAT16500.12415985167036386No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTAAAGGAGAAAT16350.12303112574608784No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGCAGTAGAGTTCACT16050.12077367389753578No Hit
GTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACC15890.11956969957830799No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15380.11573203143576948No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15320.11528054106605906No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15310.11520529267110734No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT15210.11445280872158997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGAGAGCGCGGGCCA15130.11385082156197608No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15120.11377557316702436No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14950.11249635045284484No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14950.11249635045284484No Hit
TTAGTATATAGTTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCT14910.11219535687303789No Hit
ATATACTAATTGGCCCGCGCTCTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT14910.11219535687303789No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC14710.11069038897400318No Hit
TGGTAAGAGTGAACTCTACTGCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCAC14550.1094864146547754No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14520.10926066946992022No Hit
CTACTGCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGC14400.10835768873049938No Hit
GTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTACTGCAGACCCACTGCC14190.10677747243651294No Hit
GTCCTGCAGGAGAGGGTGGCTGTTTCCCCTGGAGACACAGACAGGGTGACCGGAGACTGCGTCACCACTATTTCT14150.106476478856706No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14060.10579924330214037No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCC14060.10579924330214037No Hit
GTCAGAGTGTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG14000.10534775293242996No Hit
GGAGAAATAGTGGTGACGCAGTCTCCGGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGG13880.10444477219300913No Hit
GCAGTAGAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAACTATATACT13580.10218732034445706No Hit
GTTCAGTGGCAGTGGGTCTGCAGTAGAGTTCACTCTTACCATCAGCAGCC13550.10196157515960184No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTAAAGGAGAAATAGTGGTGACGCAGTCTC13450.10120909121008449No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13320.10023086207571193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.048.33741
GAGCCAT9100.046.7325770
GAGCATT1206.492048E-445.7275770
AAGCGGT8750.045.46626770
TGGTATC3150.041.7167132
GTGGTAT3150.040.651641
TGACTGG21300.035.42276470
CAGTTCG21450.035.1750570
GTATCAA30600.034.9094431
TCGTATG701.0376007E-634.22096340
TACAGTT4156.002665E-1033.05607670
TATCAAC34650.030.922882
GTAGTTT1800.004754034330.48504470
CGTATGC902.1191954E-730.45704841
ACGAGAC1001.8180799E-830.337422
ATAGTTG24500.030.23618770
ATCAACG36000.029.950463
TCAACGC36750.029.3392284
CTTCTCG9450.029.03337570
CAACGCA37400.028.829325
Produced by FastQC (version 0.11.5)