FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328932
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53850.40521260681509663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT43230.32529881137635336No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTA36160.2720981961454762No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32660.24576125791236875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA29600.22273524905713762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29340.2207787907883925No Hit
GTCTCCGGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCT28820.2168658742509022No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG27820.20934103475572868No Hit
GTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGCCTGGT27660.2081370604365009No Hit
GTATATAGTTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTACTGC27520.20708358290717657No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27370.20595485698290056No Hit
ACTATATACTAATTGGCCCGCGCTCTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACC26600.20016073057161693No Hit
CTCCTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT26280.19775278193316137No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGCAGTAGAGTTC23730.17856444122046877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23570.177360466901241No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC23210.1746515246829785No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC22950.17269506641423338No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCC22300.16780392074237058No Hit
CTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT21810.1641167493897355No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21710.16336426544021815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21300.160279081247197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG21280.16012858445729353No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21260.15997808766739005No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGAG20750.15614041952485155No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC20140.15155026743279565No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19260.14492840867704293No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18710.14078974695469745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18680.14056400176984224No Hit
CACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCC18310.13777981115662802No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17910.1347698753585586No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17800.1339421430140895No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17570.13221142993019958No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC17000.12792227141795065No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16950.127546029443192No Hit
CACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGCAGTAGAGTTCACTCTTACCATCAGC16890.12709453907348156No Hit
CTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT16750.12604106154415728No Hit
CCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGGCACTTAGC16550.12453609364512254No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTAAAGGAGAAAT16280.1225043869814257No Hit
CTGCAGTAGAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAACTATAT16090.1210746674773427No Hit
CTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAACTATATACTAATTGGCCCGCG15870.11941920278840452No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15840.11919345760354931No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15710.11821522846917674No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT15520.11678550896509376No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15320.11528054106605906No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15210.11445280872158997No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15200.11437756032663822No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14850.11174386650332749No Hit
GTTTACAGGCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACC14730.11084088576390665No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGCAGTAGAGTTCACT13980.10519725614252648No Hit
TTAGTATATAGTTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCT13930.1048210141677678No Hit
CTACTGCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGC13930.1048210141677678No Hit
GTCCTGCAGGAGAGGGTGGCTGTTTCCCCTGGAGACACAGACAGGGTGACCGGAGACTGCGTCACCACTATTTCT13880.10444477219300913No Hit
GTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTACTGCAGACCCACTGCC13730.10331604626873309No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGAGAGCGCGGGCCA13680.10293980429397441No Hit
TGGTAAGAGTGAACTCTACTGCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCAC13620.10248831392426398No Hit
ATATACTAATTGGCCCGCGCTCTCTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT13290.10000511689085671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)