FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063445
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48610.4570993328286841No Hit
GTACATACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT43230.4065090343177127No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT37240.3501826610685085No Hit
GCGTATGTACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT36790.3459511305239105No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACC35660.33532528715636445No Hit
GTAATATACGGCCGTGTCCTCGTCTCTCAGGGTGTTCATTTGCAGATACAGGGTGTTCTTGGAATTGTCTCTGG32320.30391792711423726No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30130.2833244784638604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27640.2599100094504182No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT26170.24608700967139815No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25390.2387523567274283No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25230.23724781253379348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24180.22737424126306485No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCAACTACGTAGTCTGGT22690.21336317345984043No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGCCAACTACGTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT22390.21054215309677513No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21720.20424187428592921No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG21330.20057454781394432No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGCCA20970.19718932337826592No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCCGTGGCCCTGTT20050.1885381942648656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19990.18797399019225255No Hit
GTACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT19920.18731575210753731No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTATGTAC19920.18731575210753731No Hit
GTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACGGGCATC19710.18534103785339157No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA19670.18496490180498287No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19120.1797930311393631No Hit
GGCCAGTACATACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTC18200.1711419020259628No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC17740.16681633746926264No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17590.16540582728772996No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG17500.16455952117881037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17220.1619265688399494No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC16990.1597637865615993No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16940.15929361650108845No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTACATACGCTG16920.15910554847688407No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTATGTACTGG16780.1577890723074536No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16690.156942766198534No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCC16580.15590839206541007No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15510.1458467527704771No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15420.14500044666155748No Hit
GTGTAGTATGTGTCAGTACCTCCAGGGTTCAGAGTTGAGACCCACTGCAGCCCCCTCCCTGGAGCCTGGCGGACC15250.14340186845582045No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTTTTTTAAAAGGTGCCC15240.1433078344437183No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGAAGTTGTGTTGACGCAGTCTCCAGCCACCCTGTCTTTGTCTCC15240.1433078344437183No Hit
GTTTATTACTGTCAGCAGCGTATGTACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA15220.14311976641951393No Hit
ATACTACACAGACTCCGTGAAGGGCCGGTTCAGCATCTCCAGAGACAATT15020.14123908617747039No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14730.13851209982650725No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC14600.13728965766917894No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14590.13719562365707677No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13910.13080131083412871No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCCGTGGCCCTGTTGGATGCATCAT13910.13080131083412871No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAACTATGCCTTGGCCTGGGTCCGCCAGGCTCCAGGGAGGGGG13500.12694591633793945No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13410.12609961022901983No Hit
TCTATGATGCATCCAACAGGGCCACGGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13190.12403086196277194No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13140.12356069190226104No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT13020.12243228375703491No Hit
GTGTGAGGTGCAACTGTTGGAGTCTGGGGGAGGCTTGGTGCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC12970.12196211369652403No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12920.12149194363601315No Hit
GTCAGAGTGTTGCCAACTACGTAGTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG12800.12036353549078702No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12730.11970529740607178No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC12630.11876495728504999No Hit
GTATATTACTGTGCGACAGCAGACGGTTCGTATATGAATATGATCTTTGACAACTGGGGCCAGGGAACCCTGGTC12540.11791865117613042No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC12490.11744848111561951No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12440.11697831105510863No Hit
GTACCAGACTACGTAGTTGGCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAA12430.11688427704300645No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11950.11237064446210195No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11740.11039593020795621No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11710.11011382817164969No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11610.1091734880506279No Hit
CATCTATGATGCATCCAACAGGGCCACGGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC11540.10851524996591266No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC11530.10842121595381049No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACCGGAGAAGTTGTGTT11370.10691667176017565No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11010.10353144732449727No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10900.10249707319137333No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC10850.10202690313086243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT405.0886854E-4101.469370
CTAATGG809.378634E-567.646270
GTGGTAT2400.056.46741
TGGTATC2850.047.5156942
CAGTTCG19100.045.3335870
GGTATCA9150.043.6928061
ATTCCGG5900.043.5687470
GGTGAGT3705.456968E-1240.22206570
GCAAATG9800.040.0355170
GCAGCGT20600.038.74879570
GTATCAA23100.036.960481
GCTTGAC754.715548E-836.1391331
AAATATT1500.002081977436.07797670
AAAACCG1555.2438304E-434.9141770
AGAGTCG400.002855199933.83741811
TCTCGCG5600.033.823170
GTCGAGG10100.033.48821670
AAGCGGT7950.032.33403470
TAATCCG2102.7790834E-432.21247570
TATCAAC26500.032.1941222
Produced by FastQC (version 0.11.5)