FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004693032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116962
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTTCCGGCAGAAACCAGGACAGCCTCCTAAGTTGGTC72660.6505145206372285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT62510.5596430317235501No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60990.5460346905266249No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCCGGAACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT51970.46527992895013437No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGAGACAGCCAGGGACTCTGG50670.45364121608434305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43710.39132933797210645No Hit
CTCCTAAGTTGGTCATTTACTGGGCATCTAACCGGGAATCCGGGGTCCCT41650.3728864545078525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA39630.354801685285623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG39420.35292158551499514No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36640.32803264569430296No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG35980.3221237607009012No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35440.3172892184335725No Hit
ATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACGA33410.29911492065083684No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33370.29875680640881247No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC32680.2925793357338925No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA30360.27180870969648024No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30320.27145059545445593No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG29960.2682275672762368No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC29510.26419878205346287No Hit
AACCAGGACAGCCTCCTAAGTTGGTCATTTACTGGGCATCTAACCGGGAA29340.2626767965248594No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28710.2570364972129759No Hit
ATATTATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCAT27370.24503967010516023No Hit
GAGTATCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26460.23689257109910633No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25260.2261491438383759No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC25160.225253858233315No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC24720.22131460157104715No Hit
GTTTATTACTGTCACCAATATTATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACGA24560.21988214460294978No Hit
GTCCTGGTTTCTGCCGGAACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACTCTGGCTGGACTTGC24420.21862874475586458No Hit
CTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG24290.21746487346928542No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24190.2165695878642246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23620.2114664599153776No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTGTACGGAGTATCATAATATTGGTGAC23420.2096758887052559No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22630.20260313242527497No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC22470.2011706754571776No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT22410.20063350409414105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22280.19946963280756194No Hit
GTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCA21860.19570943326630627No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21080.18872620554683148No Hit
GTAAATGACCAACTTAGGAGGCTGTCCTGGTTTCTGCCGGAACCAAGCTA20800.18621940585266106No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTGTACGGAGTATC20800.18621940585266106No Hit
GTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTAT20730.18559270592911845No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20500.18353354903747843No Hit
GTCAGGGACCCCGGATTCCCGGTTAGATGCCCAGTAAATGACCAACTTAGGAGGCTGTCCTGGTTTCTGCCGGA20480.18335449191646627No Hit
GGCCAAAAGTGTACGGAGTATCATAATATTGGTGACAGTAATAAACTGCC20420.18281732055342975No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20420.18281732055342975No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20280.18156392070634453No Hit
CTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTG19150.1714471933691567No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19100.17099955056662625No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18880.16902992223549235No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT18790.16822416519093755No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18790.16822416519093755No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18410.16482207989170625No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC18320.16401632284715148No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT18270.16356868004462102No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18250.16338962292360887No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATGATACT18240.16330009436310278No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACC17600.1575702664907132No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17230.154257709751988No Hit
TTATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT16660.149154581803141No Hit
ACTTAGCTTGGTTCCGGCAGAAACCAGGACAGCCTCCTAAGTTGGTCATT16610.14870693900061058No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16590.14852788187959842No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT16540.14808023907706797No Hit
GGATCTCTGGTGCCTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGG16230.14530485370137927No Hit
TCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15980.1430666396887271No Hit
CTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATAC15770.14118653991809926No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15740.140917954236581No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15330.13724728325583144No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15000.13429284075913056No Hit
GTATCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14860.13303944091204536No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14810.1325917981095149No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTATGGGGACATCGTAATG14490.12972688417332012No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14430.1291897128102836No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14300.12802584152370447No Hit
CTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTA14240.12748867016066795No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC14220.12730961303965577No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14100.12623527031358273No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14090.12614574175307666No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13990.12525045614801578No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGTTAGATGCCCAGTAAAT13980.12516092758750968No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT13800.12354941349840011No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13760.12319129925637577No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG13710.12274365645384534No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAAT13680.12247507077232708No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATGATACTCCGTACACTTTTGGCCAGGGGACCACG13620.12193789940929056No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13610.12184837084878448No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13600.12175884228827838No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC13510.1209530852437236No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC13430.1202368567596749No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13420.12014732819916882No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG13390.11987874251765056No Hit
GTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCC13340.11943109971512013No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC13260.11871487123107141No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13250.11862534267056535No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC13100.11728241426297403No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13000.11638712865791316No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTATGGGGACATCGT12800.11459655744779142No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC12730.11396985752424882No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12710.11379080040323664No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12420.11119447214856013No Hit
GCCTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCA12310.11020965798299316No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTTCCGGC12270.10985154374096881No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC12170.10895625813590795No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT12080.10815050109135316No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG11890.1064494584417375No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11850.10609134419971315No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11850.10609134419971315No Hit
CCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATAC11800.10564370139718271No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11790.10555417283667663No Hit
GTGTACGGAGTATCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAG11780.10546464427617054No Hit
TTATTACTGTCACCAATATTATGATACTCCGTACACTTTTGGCCAGGGGA11760.10528558715515836No Hit
CAATAAGAACTACTTAGCTTGGTTCCGGCAGAAACCAGGACAGCCTCCTAAGTTGGTCATTTACTGGGCATCTA11720.10492747291313402No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGC11720.10492747291313402No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT11650.10430077298959142No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG11470.10268925890048183No Hit
GCTCTGGATCTCTGGTGCCTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCG11300.10116727337187836No Hit
CTTAGGAGGCTGTCCTGGTTTCTGCCGGAACCAAGCTAAGTAGTTCTTAT11240.10063010200884184No Hit
GTCATTTACTGGGCATCTAACCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTC11190.10018245920631141No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTC11190.10018245920631141No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11170.10000340208529925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)