FastQCFastQC Report
Wed 8 May 2024
EGAF00004693033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116962
Total Bases81.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTTCCGGCAGAA70360.6299229517208285No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT67070.6004680553143258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62410.5587477461184893No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCCGGAACCAAGCTAAGTAGTTCTTA51800.46375794342153087No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA49390.4421815603395639No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47530.42552924808543174No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45230.4049376791690317No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43980.3937466091057708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42560.3810335535139065No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40220.3600838703554821No Hit
CTCCTAAGTTGGTCATTTACTGGGCATCTAACCGGGAATCCGGGGTCCCT39010.34925091453424556No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35760.3201541323697673No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35050.3137976045738351No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34370.3077096624594212No Hit
ATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAACGA33220.29741387800122115No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32900.29454896406502634No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32520.2911468787657951No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32280.28899819331364895No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC31380.2809406228681012No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA30440.272524938180529No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA30140.2698390813653464No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30060.26912285288129767No Hit
GAGTATCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGC29740.26625793894510286No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29540.26446736773498114No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29450.2636616106904264No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC28780.25766319713651853No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA28190.2523810120666594No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGTT27730.24826269828337938No Hit
ATATTATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCA27580.24691976987578806No Hit
AACCAGGACAGCCTCCTAAGTTGGTCATTTACTGGGCATCTAACCGGGAA27210.24360721313706285No Hit
GTTTATTACTGTCACCAATATTATGATACTCCGTACACTTTTGGCCAGGG27140.2429805132135202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC27110.242711927532002No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTG26780.23975748503530112No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC26750.23948889935378284No Hit
CTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCA26690.23895172799074635No Hit
GTCCTGGTTTCTGCCGGAACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG25950.23232661451329586No Hit
GTCAGGGACCCCGGATTCCCGGTTAGATGCCCAGTAAATGACCAACTTAG25780.2308046289846924No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA25500.22829782929052198No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG24420.21862874475586458No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23020.20609474628501237No Hit
GGCCAAAAGTGTACGGAGTATCATAATATTGGTGACAGTAATAAACTGCC22760.2037670037118541No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCC22620.2025136038647689No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22510.20152878969920193No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT22380.20036491841262283No Hit
GTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA22060.19750000447642801No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21650.19382933349567846No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20940.18747280569974628No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC20650.18487647744506974No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA20010.17914664957268017No Hit
GTAAATGACCAACTTAGGAGGCTGTCCTGGTTTCTGCCGGAACCAAGCTA19940.17851994964913756No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19540.17493880722889407No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19480.17440163586585755No Hit
GTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACT19360.1733272931397845No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19230.17216342185320538No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGT19130.17126813624814452No Hit
CTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTC19100.17099955056662625No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC19000.1701042649615654No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA18730.16768699382790103No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA18640.16688123678334624No Hit
GTATCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG18590.16643359398081584No Hit
TCATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT18490.16553830837575495No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18260.16347915148411496No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18010.1612409374714628No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG17910.1603456518664019No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG17430.1560482809621097No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17430.1560482809621097No Hit
GGATCTCTGGTGCCTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCC17380.15560063815957928No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17170.15372053838895147No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT17080.15291478134439668No Hit
TTATGATACTCCGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAAC16940.15166138149731145No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACC16890.15121373869478102No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16810.15049751021073232No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16330.14620013930644016No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16210.14512579658036712No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16080.14396192529378796No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT15850.14190276840214797No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT15750.1410074827970871No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATGATACTCCGT15530.13903785446595318No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15460.13841115454241057No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT15370.13760539749785577No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG15360.1375158689373497No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15290.1368891690138071No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15120.1353671834852036No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14760.13214415530698448No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA14670.1313383982624297No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14620.13089075545989926No Hit
CTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTA14600.13071169833888707No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14560.13035358409686273No Hit
CTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGC14490.12972688417332012No Hit
GTGTACGGAGTATCATAATATTGGTGACAGTAATAAACTGCCACATCTTC14300.12802584152370447No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT14240.12748867016066795No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14020.12551904182953405No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13940.12480281334548535No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA13790.12345988493789403No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT13670.122385542211821No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13530.12113214236473577No Hit
ACTTAGCTTGGTTCCGGCAGAAACCAGGACAGCCTCCTAAGTTGGTCATT13390.11987874251765056No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13320.11925204259410793No Hit
GTCATTTACTGGGCATCTAACCGGGAATCCGGGGTCCCTGACCGATTCAG13290.11898345691258969No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13250.11862534267056535No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13250.11862534267056535No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGTTAGATGCCCAGTAAAT13180.11799864274702272No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12980.11620807153690099No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12970.1161185429763949No Hit
GGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA12910.11558137161335838No Hit
GCCTATGGGGACATCGTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTC12800.11459655744779142No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12800.11459655744779142No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA12590.11271645767716358No Hit
CCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGCCACCATCAACTGCAA12530.11217928631412706No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12480.11173164351159665No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12260.10976201518046272No Hit
CTCCAGCGTGGTCCCCTGGCCAAAAGTGTACGGAGTATCATAATATTGGT12250.10967248661995664No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGTTAGATGCCCAGTAAATGACCA12190.10913531525692012No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12120.1085086153333775No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT12030.10770285828882271No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT11910.10662851556274967No Hit
TTATTACTGTCACCAATATTATGATACTCCGTACACTTTTGGCCAGGGGA11850.10609134419971315No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAACAATAA11800.10564370139718271No Hit
GTAATGACCCAGTCTCCAGAGTCCCTGGCTGTCTCTCTGGGCGAGAGGGC11620.10403218730807315No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11580.1036740730660488No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11550.10340548738453054No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11440.10242067321896359No Hit
CTTAGGAGGCTGTCCTGGTTTCTGCCGGAACCAAGCTAAGTAGTTCTTAT11420.10224161609795142No Hit
GCTCTGGATCTCTGGTGCCTATGGGGACATCGTAATGACCCAGTCTCCAG11310.10125680193238445No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11260.10080915912985401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)