FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004693034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336831
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGAATTGTCTCTGG45360.3393099052909455No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37970.28402991851625226No Hit
ATGTATTACTGTGCGACAGAGGATTTTGGAGGCCCTACTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTC32670.2443839198821691No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30210.22598219221427387No Hit
GCATGGGACAGCAGCCTCAGTGGTTGGGTGTTCGGCGGAGGAACCAAGGTGACCGTCCTCGGTCAGCCCAAGG29110.21775377740342644No Hit
CACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGTTTGGCTGCAGCAGCACCAGGGCCACCCTCC27600.20645840798126316No Hit
GCCTTGGGCTGACCGAGGACGGTCACCTTGGTTCCTCCGCCGAACACCCAACCACTGAGGCTGCTGTCCCATG26020.19463941216204592No Hit
GTCCCATGCTGAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC25670.19202128017677628No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25520.19089922361166073No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG24250.1813991446936823No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTATTGCTCAGCATGG23560.17623768449415073No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC23340.17459200153198123No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG20970.15686350780315536No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA20820.1557414512380398No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18980.14197755737262227No Hit
CTCCTGTGCAGCGTCTGGATTAACCTTCAATAACTATGTCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG18790.1405562857234759No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA18410.13771374242518314No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAAT18340.13719011602812922No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17330.1296349351563511No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG17280.1292609163013126No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17220.12881209367526636No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16900.12641837300301983No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16840.1259695503769736No Hit
CTCCTATCCTTCAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTTTCTGCATCCAGGTCAGGAAACAC16660.12462308249883494No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT15980.11953642607031105No Hit
GTATTACTGTGCGACAGAGGATTTTGGAGGCCCTACTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCA15930.11916240721527252No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15710.11751672425310304No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG15700.11744192048209535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC15410.11527261112287193No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15260.11415055455775637No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14780.11055997354938657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14720.11011115092334034No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14680.10981193583930954No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTTCCTC14510.10854027173217856No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG14500.10846546796117085No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14430.10794184156411692No Hit
GGGCTGGAGTGGGTGGCACTTATTTGGTATGATGGAAATAGAATAGACTATGCAGACTCCGGAAAGGGCCGATTC13860.1036780266166778No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC13650.10210714742551602No Hit
GACTATTATTGCTCAGCATGGGACAGCAGCCTCAGTGGTTGGGTGTTCGG13500.10098509086040046No Hit
GAATAGACGTTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGTAGGGCCTCCAAAATCCTCTGTCGC13470.10076067954737736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2750.077.30118670
GGATGGG14300.068.20692470
TACGGAA150.002560346967.56489615
TACGTAT851.8698999E-458.8451970
ACAGACG9650.053.1283770
GCTCGGG5250.047.63658570
GTGAGCG6850.045.63723870
GTGGTAT3450.044.0723041
GCCGGTT14500.043.9817170
CTAATGG1200.001024534941.68201470
CCTGCTT11150.041.49509470
GGTATCA14050.039.9212461
CGTATGC1850.038.94190641
AATCCGA350.00149457938.6085118
ACGAGAC2200.038.3905822
CGAGACG2250.037.5430723
GTATCAA35500.037.1200521
GTTTGGT1400.002185197835.72743670
AGGATGG12600.035.25893469
TCGTATG1950.035.11358640
Produced by FastQC (version 0.11.5)