FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336831
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGAATTGTCTCTGG43770.32741610570072055No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38510.28806932215066827No Hit
ATGTATTACTGTGCGACAGAGGATTTTGGAGGCCCTACTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTC30250.22628140729830473No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30100.22515935073318916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28120.21034820407366375No Hit
GCATGGGACAGCAGCCTCAGTGGTTGGGTGTTCGGCGGAGGAACCAAGGTGACCGTCCTCGGTCAGCCCAAGG26330.19695832906328473No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC25740.1925449065738302No Hit
GTCCCATGCTGAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC25150.18813148408437566No Hit
GCCTTGGGCTGACCGAGGACGGTCACCTTGGTTCCTCCGCCGAACACCCAACCACTGAGGCTGCTGTCCCATG25110.18783226900034483No Hit
CACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGTTTGGCTGCAGCAGCACCAGGGCCACCCTCC24940.18656060489321388No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22280.1666628018051646No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTATTGCTCAGCATGG21470.16060369635354058No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA20160.15080440235153134No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGACATAGTTATTGAAGGTTAATCCAGACGCTGCAC19250.14399725918983028No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG18830.1408555008075067No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA18790.1405562857234759No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18100.1353948255239443No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG17630.13187904828658223No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAAAAT17620.13180424451557451No Hit
CTCCTGTGCAGCGTCTGGATTAACCTTCAATAACTATGTCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG17260.12911130875929716No Hit
CTCCTATCCTTCAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTTTCTGCATCCAGGTCAGGAAACAC16830.1258947466059659No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16780.12552072775092737No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16200.12118210903248054No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15930.11916240721527252No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15880.11878838836023402No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT15760.11789074310814157No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG15730.11766633179511846No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15560.1163946676879875No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15440.11549702243589505No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15150.11332771307667162No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG15000.11220565651155606No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTTCCTC14630.10943791698427101No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14100.10547331712086269No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC14040.10502449449481648No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13810.10330400776163927No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC13740.10278038136458535No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13620.10188273611249289No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT13410.10031185692133111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)