FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957194
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG55990.5849388943098264No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50540.5280016381214258No Hit
GACCTAGGACGACCAGCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGTACAATAATAGT47110.4921677319331295No Hit
GCCTTGGGCTGACCTAGGACGACCAGCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGT42530.4443195423289322No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41540.43397681138828703No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34270.3580256457938516No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA32080.3351462712887878No Hit
GGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCAC29980.31320714505105546No Hit
CACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTC29550.30871484777380553No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTC29180.30484938267477646No Hit
GTTTAGAACCTATGAAAATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC29110.30411807846685207No Hit
TCTCTGGGCTCCAAGTTGAGGACGAGGCTGACTATTATTGTACTTCCTTT28400.2967005643579045No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGCTTGGTCCCTC28070.2932529873776894No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC27430.28656677747666615No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG26390.2757016863875035No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATGTGCAAAAAGGTTGAGTTGTTGGCGTTGTCTCTGG26020.27183622128847446No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT25860.27016466881321866No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC24910.2602398259913873No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24820.25929957772405593No Hit
CTATTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGTC23960.250314983169556No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23830.24895684678341068No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23220.24258405297149793No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGGAATTCTAACTATGTCTCCTGGTACCAACAGCCCCCAGGCAAAGC22760.23777833960513753No Hit
GTGTTGGTGTTTGCAAAGGAAGTACAATAATAGTCAGCCTCGTCCTCAAC22690.2370470353972131No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22100.23088318564470733No Hit
GTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCACCATCTCCTG21850.22827138490212015No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC21820.2279579688130097No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA21570.22534616807042251No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC21060.22001809455554464No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20790.21719734975355048No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG19820.2070635628723122No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19710.20591437054557382No Hit
GTCAATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGAATTCTAACTATGTCTCCTGGTACCAACAGCC19540.20413834604061454No Hit
TTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGTCAGCC19310.20173548935743432No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18580.19410903118907974No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18530.19358667104056232No Hit
GTACAATAATAGTCAGCCTCGTCCTCAACTTGGAGCCCAGAGATGGTCAG18410.19233300668412046No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT18050.1885720136147949No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAAAATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC18040.1884675415850914No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17890.18690046113953912No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17290.18063213935732986No Hit
CTCCTGGGCAGTCAATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGAATTCTAACTATGTCTCCTGGT16420.17154307277312647No Hit
GTTGGTACCAGGAGACATAGTTAGAATTCCCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATTGACTGCC16280.1700804643572776No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA16180.16903574406024274No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAAAATTCT16010.16725971955528346No Hit
GACCATCTCTGGGCTCCAAGTTGAGGACGAGGCTGACTATTATTGTACTT15780.16485686287210324No Hit
GTTGAGGACGAGGCTGACTATTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCA15690.16391661460477186No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAAC15560.1625584782186265No Hit
GACTATTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGT15490.16182717401070212No Hit
CAATAATAGTCAGCCTCGTCCTCAACTTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGC15240.15921537326811494No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14900.15566332425819637No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGC14830.15493202005027193No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14740.15399177178294055No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14140.1477234500007313No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14120.14751450594132434No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGC13860.14479823316903365No Hit
GTATATTACTGTGCGAGGTTTATTGTGGCATCTCGAGAGAGGTACTTTGACAAGTGGGGCCAGGGAACCTTGATC13520.14124618415911508No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACT13420.1402014638620802No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAGAGAAG13420.1402014638620802No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13360.13957463168385928No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTATTG13350.1394701596541558No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA13220.13811202326801045No Hit
GTGCAGGAGATGGTGATTGACTGCCCAGGAGACCCAGACACGGAGGCAGG13070.13654494282245813No Hit
GGTTTAGAACCTATGAAAATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC13070.13654494282245813No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC12950.1352912784660163No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12840.13414208613927792No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12630.1319481735155047No Hit
TCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGC12240.12787376435706868No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAAAATTCTGTAGGGGCC12010.12547090767388847No Hit
TCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGT11970.12505301955507453No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11950.12484407549566755No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11920.12453065940655708No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGCTTGGTC11910.1244261873768536No Hit
CCCCTGAGGGCCGATTATTGATATCATAAATTATGAGTTGGGGGGCTTTGCCTGGGGGCTGTTGGTACCAGG11800.12327699505011523No Hit
GGTACCAACAGCCCCCAGGCAAAGCCCCCCAACTCATAATTTATGATATCAATAATCGGCCCTCAGGGGTTTCTA11770.12296357896100477No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11740.12265016287189431No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11730.12254569084219082No Hit
AGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCACCATCTCCTGCACTGGAACC11550.12066519430752803No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11520.12035177821841758No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11450.11962047401049318No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG11420.11930705792138271No Hit
CTCCCTGACCATCTCTGGGCTCCAAGTTGAGGACGAGGCTGACTATTATTGTACTTCCTTTGCAAACACCAAC11400.11909811386197575No Hit
ATAATAGTCAGCCTCGTCCTCAACTTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAG11230.11732208935701645No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11220.11721761732731296No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG11110.11606842500057458No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG11010.11502370470353972No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10990.11481476064413275No Hit
GGACCAAGCTGGTCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG10840.11324768019858042No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCACCATCTCCTGCA10820.11303873613917345No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCC10780.1126208480203595No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC10540.1101135193074758No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCC10480.10948668712925488No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT10410.10875538292133048No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC10380.10844196683222002No Hit
GCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGTACAATAATAGTCAGCCTCGTCCTCA10350.10812855074310954No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGG10130.10583016608963283No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGG10100.10551675000052235No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGC10000.10447202970348748No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT9910.1035317814361561No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC9810.10248706113912122No Hit
ACCTAGGACGACCAGCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGTACAATAATAGTC9670.1010244527232724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)