FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004693043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004693043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957194
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG49870.521002012131292No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46820.48913804307172837No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39650.41423159777432783No Hit
GACCTAGGACGACCAGCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGTACAATAATAGT39090.4083811641109326No Hit
GCCTTGGGCTGACCTAGGACGACCAGCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGT39040.40785880396241514No Hit
GGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCAC35960.375681418813741No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA33420.3491455232690552No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTC31610.33023608589272396No Hit
GTTTAGAACCTATGAAAATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC28450.29722292450642185No Hit
CACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTC27860.2910590747539161No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27540.2877159698034045No Hit
TCTCTGGGCTCCAAGTTGAGGACGAGGCTGACTATTATTGTACTTCCTTT27250.2846862809420034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26940.28144764802119526No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC25800.26953783663499775No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT25730.2688065324270733No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATGTGCAAAAAGGTTGAGTTGTTGGCGTTGTCTCTGG25290.26420976312011984No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGCTTGGTCCCTC25210.263373986882492No Hit
CTATTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGTC24510.25606094480324787No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC24190.2527178398527362No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG23730.2479121264863758No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23230.24268852500120142No Hit
GTGTTGGTGTTTGCAAAGGAAGTACAATAATAGTCAGCCTCGTCCTCAAC22890.23913647599128285No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22200.2319279059417422No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC21960.22942057722885853No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21940.22921163316945153No Hit
GTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCACCATCTCCTG21090.22033151064465512No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC20700.21625710148621907No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGGAATTCTAACTATGTCTCCTGGTACCAACAGCCCCCAGGCAAAGC20160.21061561188223077No Hit
TTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGTCAGCC20130.21030219579312032No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA19680.20560095445646334No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC19420.2028846816841727No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18840.19682530396137043No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG18770.19609399975344602No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18590.19421350321878322No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18570.19400455915937626No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17870.18669151708013215No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17760.18554232475339377No Hit
CTCCTGGGCAGTCAATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGAATTCTAACTATGTCTCCTGGT17170.17937847500088802No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16530.17269226509986482No Hit
GTTGGTACCAGGAGACATAGTTAGAATTCCCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATTGACTGCC16350.17081176856520203No Hit
GTACAATAATAGTCAGCCTCGTCCTCAACTTGGAGCCCAGAGATGGTCAG16300.1702894084166846No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT16060.1677820797038009No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAAC15770.16475239084239976No Hit
GTCAATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGAATTCTAACTATGTCTCCTGGTACCAACAGCC15770.16475239084239976No Hit
GACCATCTCTGGGCTCCAAGTTGAGGACGAGGCTGACTATTATTGTACTT15660.1636031985156614No Hit
GACTATTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCAAGCTGGTCGTCCTAGGT15440.16130481386218468No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15200.15879748514930098No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAAAATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC15040.15712593267404518No Hit
GTTGAGGACGAGGCTGACTATTATTGTACTTCCTTTGCAAACACCAACACTCTGGTTTTCGGCGGAGGGACCA14710.1536783556938301No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA14650.15305152351560916No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA14070.1469921457928069No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13610.14218643242644646No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGC13490.14093276807000463No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAAAATTCT13480.14082829604030114No Hit
CAATAATAGTCAGCCTCGTCCTCAACTTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGC13470.14072382401059763No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13330.1392612155947488No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGC13230.13821649529771393No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACT12890.13466444628779536No Hit
GTATATTACTGTGCGAGGTTTATTGTGGCATCTCGAGAGAGGTACTTTGACAAGTGGGGCCAGGGAACCTTGATC12780.133515253961057No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12750.13320183787194653No Hit
TCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGC12380.1293363727729175No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12370.12923190074321403No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12360.12912742871351052No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCACCATCTCCTGCA12340.12891848465410355No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12080.12620221188181288No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12070.1260977398521094No Hit
GGTTTAGAACCTATGAAAATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC11910.1244261873768536No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC11860.12390382722833615No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAGAGAAG11820.1234859391095222No Hit
GTGCAGGAGATGGTGATTGACTGCCCAGGAGACCCAGACACGGAGGCAGG11810.12338146707981872No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11790.12317252302041173No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTATTG11670.1219188586639699No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC11400.11909811386197575No Hit
TCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGT11370.11878469777286528No Hit
CCCCTGAGGGCCGATTATTGATATCATAAATTATGAGTTGGGGGGCTTTGCCTGGGGGCTGTTGGTACCAGG11300.11805339356494085No Hit
CTCCCTGACCATCTCTGGGCTCCAAGTTGAGGACGAGGCTGACTATTATTGTACTTCCTTTGCAAACACCAAC11230.11732208935701645No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC11120.1161728970302781No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10970.11460581658472577No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10900.11387451237680137No Hit
ATAATAGTCAGCCTCGTCCTCAACTTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGGCAG10760.11241190396095253No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10700.11178507178273162No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10680.11157612772332463No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10610.11084482351540022No Hit
GGACCAAGCTGGTCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG10570.11042693539658627No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGCCTCCAAGTCTGGCAACACGGCC10450.10917327104014443No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG10440.10906879901044093No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10380.10844196683222002No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGCTTGGTC10300.1076061905945921No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10180.10635252623815027No Hit
GCTTGGTCCCTCCGCCGAAAACCAGAGTGTTGGTGTTTGCAAAGGAAGTACAATAATAGTCAGCCTCGTCCTCA10080.10530780594111538No Hit
AGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCAATCACCATCTCCTGCACTGGAACC10060.1050988618817084No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT9940.10384519752526657No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAAAATTCTGTAGGGGCC9940.10384519752526657No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGACCAGCTTGGTCCCTCCGCCGAAAAC9920.10363625346585958No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG9800.10238258910941773No Hit
ACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGCCCCGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCA9710.10144234084208635No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCAGAGGGTCCTGGGCCCAGTCTGC9670.1010244527232724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)